Nucleoredoxin2 (NRX2) Promotes Jasmonate-Mediated Trichome Formation in Arabidopsis
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RESEARCH ARTICLE
Nucleoredoxin2 (NRX2) Promotes Jasmonate‑Mediated Trichome Formation in Arabidopsis Eun Seon Lee1 · Joung Hun Park1 · Gwang Yong Hwang1 · Yong Hun Chi2 · Chang Ho Kang1 · Ho Byoung Chae1 · Seol Ki Paeng1 · Seong Dong Wi1 · Su Bin Bae1 · Kieu Anh Thi Phan1 · Sang Yeol Lee1 Received: 6 May 2020 / Revised: 9 August 2020 / Accepted: 24 August 2020 © Korean Society of Plant Biologist 2020
Abstract Thioredoxin (Trx) proteins are essential for the maintenance of cellular redox balance through thiol/disulfide exchange modification. In Arabidopsis, the Trx superfamily consists of multiple protein isotypes distributed in most cellular compartments. Although the functions of chloroplastic and cytosolic Trxs have been investigated in plants, the physiological role of nuclear Trx proteins remains elusive. Nucleoredoxin (NRX) is a nuclear Trx first identified in eukaryotic organisms. Arabidopsis possesses two NRX genes (AtNRX1 and AtNRX2), and the function of AtNRX2 has not been elucidated to date. In this study, we characterized the function of AtNRX2 using the atnrx2 knockout mutant, based on its comparison with the atnrx1 mutant. In atnrx2 knockout mutant plants, trichome number was significantly reduced compared with the wild type (WT; Col-0) and the atnrx1 mutant. In response to JA induction of trichome, trichome formation was markedly diminished in the atnrx2 mutant. In addition, expression levels of genes involved in trichome formation were reduced in the atnrx2 mutant compared with the WT and atnrx1 mutant. Overall, our results suggest that AtNRX2 plays a physiological role in JA-mediated trichome formation in Arabidopsis. Keywords Arabidopsis · Jasmonate · Nucleoredoxin · Redox protein · Trichome
Introduction Reactive oxygen species (ROS) act as a secondary messenger in plants, and ROS homeostasis is critical for the maintenance of normal growth and development in all living organisms. In plants, like calcium, cAMP, and other signaling molecules, ROS modulate diverse signaling cascades to maintain normal growth, metabolism, and stress responses by mediating protein dithiol–disulfide redox modification. However, excessive Eun Seon Lee and Joung Hun Park contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12374-020-09277-z) contains supplementary material, which is available to authorized users. * Sang Yeol Lee [email protected] 1
Division of Applied Life Science (BK21+) and PMBBRC, Gyeongsang National University, Jinju 52828, Korea
Plant Propagation Team, Plant Production Division, Sejong National Arboretum, Sejong 30106, Korea
2
accumulation of ROS causes oxidative stress, which results in the loss of cellular function, leading to abnormal physiology and ultimately cell death or apoptosis (Asada 2006). To maintain ROS homeostasis, plants employ a variety of antioxidant enzymes, such as catalase (CAT), protein disulifide isomerase (PDI), glutaredoxin (Grx), peroxiredoxin (Prx), and thioredoxin (Trx) (Meyer et al.
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