The RNA surveillance factor UPF1 regulates the migration and adhesion of porcine skeletal muscle satellite cells
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ORIGINAL RESEARCH
The RNA surveillance factor UPF1 regulates the migration and adhesion of porcine skeletal muscle satellite cells Yanjie Tan1 · Yi Jin1 · Sheng Wang1 · Jianhua Cao1 · Zhuqing Ren1,2
© Springer Nature Switzerland AG 2020
Abstract Skeletal muscle satellite cells (SCs) play an important role in the repairment and regeneration of damaged muscle. The activation, proliferation, migration, and differentiation of SCs are essential to the response to muscle injury. Up-frameshift 1 (UPF1) is involved in the regulation of many developmental processes. However, the role of UPF1 and its associated regulatory mechanism in SCs are still unclear. Here, we analyzed changes in the transcriptome of porcine SCs with UPF1 knockdown. The results showed that focal adhesion and actin cytoskeleton processes were regulated by UPF1. We also confirmed experimentally that UPF1 promoted SC migration and adhesion by regulating the expression of F-Actin, Vinculin, and several adhesion-related genes. Furthermore, we found that phosphorylated focal adhesion kinase (p-FAK) was down-regulated by UPF1 knockdown. This study identifies the role of UPF1 in regulating SC migration and adhesion and therefore provides new insight into the regulatory mechanism of UPF1 in the process of repairing damaged muscle. Keywords UPF1 · Skeletal muscle satellite cells · Focal adhesion · Cytoskeleton Abbreviations SCs Skeletal muscle satellite cells NMD Nonsense mediated mRNA decay Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10974-020-09585-4) contains supplementary material, which is available to authorized users. * Zhuqing Ren [email protected] Yanjie Tan [email protected] Yi Jin [email protected] Sheng Wang [email protected] Jianhua Cao [email protected] 1
Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education & Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People’s Republic of China
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, People’s Republic of China
2
SMD Staufen1 mediated mRNA decay DEGs Differentially expressed genes UPF1 Regulator of nonsense transcripts FDR False Discovery Rate
Introduction Cell migration and adhesion are important components of many biological processes, including cell growth, embryonic development, and wound healing (Ratheesh et al. 2015; Wang et al. 2016). Skeletal muscle satellite cells (SCs), a type of adult stem cell, possess a remarkable capacity to repair and regenerate injured muscle (Dumont et al. 2015). When a muscle is injured, SCs can be activated and then proliferate and migrate toward the injured site to carry out cellular tissue repair (Griffin et al. 2010; Le Grand and Rudnicki 2007). The formation of mature focal adhesion
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