The role of broth enrichment in Staphylococcus aureus cultivation and transmission from the throat to newborn infants: r
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ARTICLE
The role of broth enrichment in Staphylococcus aureus cultivation and transmission from the throat to newborn infants: results from the Swedish hygiene intervention and transmission of S. aureus study S. Mernelius & S. Löfgren & P.-E. Lindgren & A. Matussek
Received: 12 April 2013 / Accepted: 20 June 2013 / Published online: 2 July 2013 # The Author(s) 2013. This article is published with open access at Springerlink.com
Abstract Staphylococcus aureus is detected by direct plating, whereas incubation in enrichment broth prior to plating to increase the proportion of positive samples has not been fully evaluated. S. aureus throat colonization has been suggested to be more common than colonization of the anterior nares, but no data are available on the transmission of S. aureus from the throat. Swab samples were collected from the anterior nares and umbilicus from newborn infants (n=168), anterior nares, throat, skin lesions, and vagina from parents (n=332), and anterior nares, throat, and skin lesions from healthcare workers (n=231) at three maternity wards. spa typing was used to elucidate the transmission routes of S. aureus. The use of enrichment broth prior to plating increased the proportion of positive samples by 46 %. The prevalence of S. aureus colonization in adults was 58 %. Throat colonization (47 %) was significantly more common than colonization in any of the other screened sites (p
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