The Role of lncRNA TUG1 in the Parkinson Disease and Its Effect on Microglial Inflammatory Response
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ORIGINAL PAPER
The Role of lncRNA TUG1 in the Parkinson Disease and Its Effect on Microglial Inflammatory Response Jiang Cheng1 · Yangyang Duan2 · Fengting Zhang2 · Jin Shi2 · Hui Li3 · Feng Wang4 · Haining Li1 Received: 8 September 2020 / Accepted: 12 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Parkinson’s disease (PD) is a common neurodegenerative disease in the middle-aged and elderly populations. The purpose of this study was to investigate the clinical value of lncRNA TUG1 in PD and its effect on the microglial inflammatory response. A total of 181 subjects were recruited for the study, including 97 patients with PD (male/female 50/47) and 84 healthy individuals (male/female 41/43). There was no significant difference for gender and age distribution between the groups. The expression of serum TUG1 was determined by qRT-PCR. The receiver operating curve (ROC) was applied for diagnostic value analysis. CCK-8 was used to detect the effect of TUG1 on the proliferation of BV2 cells. The motor coordination ability of mice was tested by the rotarod and pole tests. ELISA was used to detect serum pro-inflammatory factors. TUG1 was highly expressed in the serum of PD patients. Serum TUG1 can distinguish PD patients to form healthy controls with the AUC of 0.902. Serum TUG1 was positively correlated with the levels of UPDRS, IL-6, IL-1β, and TNF-α in PD patients. Cell experiment results showed that the downregulation of TUG1 significantly inhibited cell proliferation and the release of TNF-α, IL-6, and IL-1β. Besides, animal experiments suggested that the downregulation of TUG1 significantly improved the motor coordination ability of the PD mice and inhibited the expression of inflammatory factors. lncRNA TUG1 is a latent biomarker of PD patients. TUG1 downregulation may inhibit the inflammatory response in the progression of PD. These findings provide a possible target for the early diagnosis and therapeutic intervention of PD. Keywords Parkinson’s disease · LncRNA TUG1 · Microglial · Inflammatory · Diagnosis
Introduction Jiang Cheng and Yangyang Duan contributed equally to the work. * Feng Wang [email protected] * Haining Li [email protected] 1
Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, NO.804 Shengli Street, Xingqing District, Yinchuan 750004, Ningxia, China
2
School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia, China
3
Department of Computer Science, Jiangsu Ocean University, Lianyungang 222000, Jiangsu, China
4
Department of Neurosurgery, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, NO.804 Shengli Street, Xingqing District, Yinchuan 750004, Ningxia, China
Parkinson’s disease (PD) is a common neurodegenerative disease, second only to Alzheimer’s disease in the world (Hugh-Jones et al. 2020). The prev
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