The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and
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BioMed Central
Open Access
Research
The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects DL Mager*1, AD Haffajee1, PM Devlin2, CM Norris3, MR Posner3 and JM Goodson1 Address: 1The Forsyth Institute, 140 The Fenway, Boston, MA, USA, 2Brigham and Women's Hospital, 27 Francis Street, Boston, MA, USA and 3Dana Farber Cancer Institute, 44 Binney Street, Boston, MA, USA Email: DL Mager* - [email protected]; AD Haffajee - [email protected]; PM Devlin - [email protected]; CM Norris - [email protected]; MR Posner - [email protected]; JM Goodson - [email protected] * Corresponding author
Published: 7 July 2005 Journal of Translational Medicine 2005, 3:27
doi:10.1186/1479-5876-3-27
Received: 22 February 2005 Accepted: 7 July 2005
This article is available from: http://www.translational-medicine.com/content/3/1/27 © 2005 Mager et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Oral Squamous Cell CarcinomaOral mucosabacterial markersbacteriaearly detection
Abstract Background: The purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (OSCC) lesion would differ from those found in cancer-free (OSCC-free) controls. Methods: Unstimulated saliva samples were collected from 229 OSCC-free and 45 OSCC subjects and evaluated for their content of 40 common oral bacteria using checkerboard DNADNA hybridization. DNA counts per ml saliva were determined for each species, averaged across subjects in the 2 subject groups, and significance of differences between groups determined using the Mann-Whitney test and adjusted for multiple comparisons. Diagnostic sensitivity and specificity in detection of OSCC by levels of salivary organisms were computed and comparisons made separately between a non-matched group of 45 OSCC subjects and 229 controls and a group of 45 OSCC subjects and 45 controls matched by age, gender and smoking history. Results: Counts of 3 of the 40 species tested, Capnocytophaga gingivalis, Prevotella melaninogenica and Streptococcus mitis, were elevated in the saliva of individuals with OSCC (p < 0.001). When tested as diagnostic markers the 3 species were found to predict 80% of cancer cases (sensitivity) while excluding 83% of controls (specificity) in the non-matched group. Diagnostic sensitivity and specificity in the matched group were 80% and 82% respectively. Conclusion: High salivary counts of C. gingivalis, P. melaninogenica and S. mitis may be diagnostic indicators of OSCC.
Background Each year nearly 30,000 Americans are diagnosed with
oral cancer. 90% of these lesions are oral squamous cell carcinomas [1]
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