The soybean gene Gm Hsp 22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana
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RESEARCH ARTICLE
Open Access
The soybean gene GmHsp22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana Suellen Mika Hishinuma-Silva1, Valéria Stefania Lopes-Caitar2, Rafael Bruno Guayato Nomura1, Bruna Caroline Sercero3, Aline Garcia da Silva4, Mayra Costa da Cruz Gallo De Carvalho4, Ivani de Oliveira Negrão Lopes5, Waldir Pereira Dias5 and Francismar Corrêa Marcelino-Guimarães5*
Abstract Background: Small heat shock proteins (sHSPs) belong to the class of molecular chaperones that respond to biotic and abiotic stresses in plants. A previous study has showed strong induction of the gene GmHsp22.4 in response to the nematode Meloidogyne javanica in a resistant soybean genotype, while repression in a susceptible one. This study aimed to investigate the functional involvement of this small chaperone in response to M. javanica in Arabidopsis thaliana. First, it was evaluated the activation of the promoter region after the nematode inoculation, and the occurrence of polymorphisms between resistant and susceptible re-sequenced soybean accessions. Then functional analysis using A. thaliana lines overexpressing the soybean GmHsp22.4 gene, and knocked-out mutants were challenged with M. javanica infestation. Results: High expression levels of the GFP gene marker in transformed A. thaliana plants revealed that the promoter region of GmHsp22.4 was strongly activated after nematode inoculation. Moreover, the multiplication of the nematode was significantly reduced in plants overexpressing GmHsp22.4 gene in A. thaliana compared to the wild type. Additionally, the multiplication of M. javanica in the A. thaliana mutants was significantly increased mainly in the event athsp22.0–2. This increase was not that evident in the event athsp22.0–1, the one that preserved a portion of the promoter region, including the HSEs in the region around − 83 bp. However, structural analysis at sequence level among soybean resistant and susceptible genotypes did not detect any polymorphisms in the whole gene model. (Continued on next page)
* Correspondence: [email protected] 5 Department of Plant Biotechnology, Brazilian Agricultural Research Corporation EMBRAPA Soybean, Londrina, PR, Brazil Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain p
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