The sugarcane ShMYB78 transcription factor activates suberin biosynthesis in Nicotiana benthamiana
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The sugarcane ShMYB78 transcription factor activates suberin biosynthesis in Nicotiana benthamiana Raquel Figueiredo1,5 · Juan Pablo Portilla Llerena1 · Eduardo Kiyota1 · Sávio Siqueira Ferreira2 · Bárbara Rocha Cardeli1 · Sarah Caroline Ribeiro de Souza1,6 · Michael dos Santos Brito3 · Ladaslav Sodek1 · Igor Cesarino2 · Paulo Mazzafera1,4 Received: 12 March 2020 / Accepted: 6 August 2020 © Springer Nature B.V. 2020
Abstract Key message A sugarcane MYB present in the culm induces suberin biosynthesis and is involved both with fatty acid and phenolics metabolism. Abstract Few transcription factors have been described as regulators of cell wall polymers deposition in C4 grasses. Particularly, regulation of suberin biosynthesis in this group of plants remains poorly understood. Here, we showed that the sugarcane MYB transcription factor ShMYB78 is an activator of suberin biosynthesis and deposition. ShMYB78 was identified upon screening genes whose expression was upregulated in sugarcane internodes undergoing suberization during culm development or triggered by wounding. Agrobacterium-mediated transient expression of ShMYB78 in Nicotiana benthamiana leaves induced the ectopic deposition of suberin and its aliphatic and aromatic monomers. Further, the expression of suberin-related genes was induced by ShMYB78 heterologous expression in Nicotiana benthamiana leaves. ShMYB78 was shown to be a nuclear protein based on its presence in sugarcane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMYB78 activates the promoters of the sugarcane suberin biosynthetic genes β-ketoacyl-CoA synthase (ShKCS20) and caffeic acid-O-methyltransferase (ShCOMT). Our results suggest that ShMYB78 may be involved in the transcriptional regulation of suberin deposition, from fatty acid metabolism to phenylpropanoid biosynthesis, in sugarcane internodes. Keywords C4 grasses · Lipid metabolism · MYB transcription factor · Phenylpropanoid metabolism · Suberin · Sugarcane
Introduction Worldwide, the growing recognition for low-carbon and renewable resources is shifting the attention from an oilbased economy towards a sustainable, bio-based economy Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01048-1) contains supplementary material, which is available to authorized users. * Raquel Figueiredo [email protected] 1
Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas 13083‑862, Brazil
2
Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo 05508‑090, Brazil
3
Institute of Science and Technology, Federal University of São Paulo, Campus São José dos Campos, São José dos Campos 12231‑280, Brazil
in which plant feedstocks are used for energy production, biofuels and bioproducts. The lignocellulosic biomass of sugarcane, a C4 bioenergy grass, is a prominent option as this plant has an impressive biomass accumulation potential being the focus of ag
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