NS3 Protein from Rice stripe virus affects the expression of endogenous genes in Nicotiana benthamiana
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RESEARCH
Open Access
NS3 Protein from Rice stripe virus affects the expression of endogenous genes in Nicotiana benthamiana Gentu Wu1, Guixian Zheng1, Qiao Hu1, Mingge Ma1, Mingjun Li1, Xianchao Sun1, Fei Yan2* and Ling Qing1*
Abstract Background: Rice stripe virus (RSV) belongs to the genus Tenuivirus. It is transmitted by small brown planthoppers in a persistent and circulative-propagative manner and causes rice stripe disease (RSD). The NS3 protein of RSV, encoded by the viral strand of RNA3, is a viral suppressor of RNA silencing (VSR). NS3 plays a significant role in viral infection, and NS3-transgenic plants manifest resistance to the virus. Methods: The stability and availability of NS3 produced by transgenic Nicotiana benthamiana was investigated by northern blot analysis. The accumulation of virus was detected by western blot analysis. Transcriptome sequencing was used to identify differentially expressed genes (DEGs) in NS3-transgenic N. benthamiana. Results: When the host plants were inoculated with RSV, symptoms and viral accumulation in NS3-transgenic N. benthamiana were reduced compared with the wild type. Transcriptome analysis identified 2533 differentially expressed genes (DEGs) in the NS3-transgenic N. benthamiana, including 597 upregulated genes and 1936 downregulated genes. These DEGs were classified into three Gene Ontology (GO) categories and were associated with 43 GO terms. KEGG pathway analysis revealed that these DEGs were involved in pathways associated with ribosomes (ko03010), photosynthesis (ko00195), photosynthesis-antenna proteins (ko00196), and carbon metabolism (ko01200). More than 70 DEGs were in these four pathways. Twelve DEGs were selected for RT-qPCR verification and subsequent analysis. The results showed that NS3 induced host resistance by affecting host gene expression. Conclusion: NS3, which plays dual roles in the process of infection, may act as a VSR during RSV infection, and enable viral resistance in transgenic host plants. NS3 from RSV affects the expression of genes associated with ribosomes, photosynthesis, and carbon metabolism in N. benthamiana. This study enhances our understanding of the interactions between VSRs and host plants. Keywords: Rice stripe virus, NS3, Suppressor of RNA silencing, Nicotiana benthamiana, Transcriptomes
Background Rice stripe virus (RSV) belongs to the genus Tenuivirus, and is characterized by transovarian transmission in a persistent, circulative-propagative manner by the small brown planthopper (Laodelphax striatellus) [1]. Since 2000, RSV has occurred in the Yangtze River region and generally caused a 30–40% reduction in yield in eastern China [2, 3]. * Correspondence: [email protected]; [email protected] 2 The State Key Laboratory Breading Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 1 Chongqing Key Laboratory of Plant Dise
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