Improved Drought and Salt Tolerance in Transgenic Nicotiana benthamiana by Overexpressing Sugarcane ScSEC14p Gene
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RESEARCH ARTICLE
Improved Drought and Salt Tolerance in Transgenic Nicotiana benthamiana by Overexpressing Sugarcane ScSEC14p Gene Yongjuan Ren1 • Huaying Mao1 • Weihua Su1 • Yachun Su1 • Chang Zhang1 • Mutian Shi1,3 • Youxiong Que1,2
Received: 28 May 2020 / Accepted: 11 September 2020 Ó Society for Sugar Research & Promotion 2020
Abstract Plant phosphatidylinositol transfer proteins (PITPs) are involved in several cellular processes such as lipid metabolism, signal transduction, osmotic regulation, protein transport and stress response. In this study, the effects of sugarcane PITP gene ScSEC14p (accession no. MH899751) on drought and salt tolerance of transgenic Nicotiana benthamiana were evaluated along with the underlying mechanisms. The results showed that ScSEC14p gene effectively improved drought and salt tolerance of transgenic lines of N. benthamiana, as indicated by the increased germination rate and superoxide dismutase levels, as well as the decreased malondialdehyde level. In addition, the enhanced tolerance was closely related to the upregulation of stress-related genes and key genes in phosphatidylinositol pathway. It is revealed that the overexpression of ScSEC14p gene resulted in improved
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12355-020-00895-y) contains supplementary material, which is available to authorized users. & Mutian Shi [email protected] & Youxiong Que [email protected] 1
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, College of Agriculture, Fujian Agriculture and Forestry University, ShangXiadian Road Num. 15, Cangshan District, Fuzhou City 350002, Fujian Province, China
2
Key Laboratory of Ministry of Education for Genetic, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
3
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
drought and salt tolerance of transgenic tobacco and also elucidated the underlying regulation mechanism by ScSEC14p in sugarcane. Keywords Phosphatidylinositol transfer proteins (PITPs) Drought tolerance Salt tolerance Osmotic stress Transgenic tobacco
Introduction Sugarcane (Saccharum spp.) is an important sugar crop. Cane sugar accounts for more than 80% of the sugar consumption worldwide and more than 90% of sugar production in China (Que et al. 2014a). However, drought stress, high salinity and osmotic stress resulted in decreased sugar content and seriously restrict crop growth and yield (Koehler et al. 1982; Gandonou et al. 2008; Begcy and Dresselhaus 2018). Plants evolve adaptive strategies against environmental stress and use complex signaling networks to tolerate cellular dehydration (Chu et al. 2015). Phospholipids are basic elements of bi-layer cell membranes and represent important components of signal transduction (Cowan 2006). The phospholipid distribution determines the physical properti
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