Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections
- PDF / 1,721,960 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 51 Downloads / 155 Views
Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections Árpád Dandé1 · Béla Kocsis2 · Dénes Lőrinczy3 Received: 7 August 2019 / Accepted: 19 December 2019 © The Author(s) 2020
Abstract The analysis of synovial fluid is an important method in diagnosing and handling septic arthritis. To achieve a quick diagnosis could be a great advantage in the therapy. The differential scanning calorimetry (DSC) proved to be a useful technique in the differential diagnosis of tumors using blood plasma or sera. The aim of this paper is to show up some characteristic thermal parameters in the diagnosis of different grades of arthritis, which are in good agreement with the severity of disease checked by conventional X-ray supported grading. To follow the effect of different bacterial strains, the synovial fluids were inoculated by three types of bacterial strains (with 1 03–105 CFU mL−1 concentrations) at 37 °C and stored trough 24 h. After that, they were denatured in 20–90 °C temperature range with 0.3 K min−1 scanning rate. The change in the maximum denaturation temperature (Tm) and calorimetric enthalpy (∆H) monitored the severity of sepsis and depended on the type of bacteria. The proliferation characteristics of bacteria should be strain specific. The synovial fluid samples inoculated with the most frequently occurring bacteria were monitored in isotherm mode (isoperibol calorimeter) at 37 °C up to the end of the proliferation. The isoperibolic scans clearly demonstrated specific, concentration-dependent representative curves in case of each bacterium (duration of proliferation, maximum of proliferation rates, etc.). Therefore, thermal analysis of human synovial fluid samples by DSC or isoperibolic calorimetry could be a useful tool in the staging of osteoarthritis and the diagnostics of septic arthritis. Keywords Synovial fluid · Septic arthritis · DSC · Isoperibolic calorimetry · Bacterial strains
Introduction In the twentieth century, with the advancement of medical science, the development of biochemical, biophysical, and electronic backgrounds brought new and more modern health care products, drugs, and technologies essential to healing. Parallel to this, in primary care as well as in hospital clinics with advanced clinical background, medical diagnostic devices and advanced surgical equipment have become commonplace in daily practice. Continuous innovation in * Dénes Lőrinczy [email protected] 1
Department of Traumatology and Hand Surgery, Clinical Center, Medical School, University of Pécs, Ifjúság str. 13, Pecs 7624, Hungary
2
Institute of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12, Pecs 7624, Hungary
3
Department of Biophysics, Medical School, University of Pécs, Szigeti str. 12, Pecs 7624, Hungary
the use of implants for locomotor surgery and the use of modern surgical techniques in the restorative surgical treatment of musculoskeletal system and in the reconstructive surgery of degenerative joint d
Data Loading...
