Comparative analysis of bacterial community and functional species in oil reservoirs with different in situ temperatures
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ORIGINAL ARTICLE
Comparative analysis of bacterial community and functional species in oil reservoirs with different in situ temperatures Yuexin Tian 1 & Shuwen Xue 1 & Yanling Ma 1 Received: 15 October 2019 / Revised: 30 March 2020 / Accepted: 2 April 2020 # Springer Nature Switzerland AG 2020
Abstract Temperature is supposed to be one of the primary drivers for the bacterial diversification as well as hydrocarbon formation process of oil reservoirs. However, the bacterial community compositions are not systematically elucidated in oil reservoirs with different temperatures. Herein, the diversity of indigenous bacteria and the functional species in the water samples from oil reservoirs with different in situ temperatures was investigated by high-throughput sequencing technology. The results showed that samples in the high (65 °C) and super high (80 °C) temperature oil reservoir had significantly high bacterial richness, even more than twice as much as moderate temperature (36 °C) ones, which showed relatively high bacterial diversity. Meanwhile, the bacterial compositions were almost similar in the high temperature oil reservoirs but there were different relative abundances of the bacterial communities. Phylogenetic analysis revealed that indigenous bacteria fell into 20 phylotypes in which Proteobacteria were the principal phylum in all of samples. At the genus level, 10 out of 22 major genera displayed statistically significant differences. Among of them, Pseudomonas was extremely dominant in all of samples, while Halomonas, Caldicoprobacter, Arcobacter, and Marinobacter tended to be enriched in the high temperature oil reservoirs. Moreover, the abundance of bacterial populations exhibited important distinction in oil reservoir such as hydrocarbon-oxidizing, fermentative, nitrate-reducing, sulfate-reducing, and methanogenic bacteria. Those bacteria were strongly correlated to in situ temperature variation. Keywords Bacterial community . 16S rRNA gene sequencing . In situ temperature . Functional species . Oil reservoirs
Introduction Crude oil is an important strategic resource related to the development of the global economy, and inadequate oil resources could elevate living costs. A significant quantum of crude oil is trapped in reservoirs after primary and secondary recoveries, which should be extracted with the steady increase in demand for oil (Mcdonald et al. 2014; Safdel et al. 2017). In the search of cost effective solutions for the extraction of trapped oil, microbial enhanced oil recovery (MEOR) has been regarded as an environmentally friendly tertiary recovery Yuexin Tian and Shuwen Xue contributed equally to this work. * Yanling Ma [email protected] 1
Shaanxi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, 229 Taibai North Rd, Xi’an 710069, Shaanxi, China
method that involves the use of microbial communities and their metabolites in retrieving residual oil (
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