Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hanseni
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Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii James González1 · Román Castillo1 · Miguel Angel García‑Campos1 · Diego Noriega‑Samaniego1 · Viviana Escobar‑Sánchez1 · Lucero Romero‑Aguilar2 · Luisa Alba‑Lois1 · Claudia Segal‑Kischinevzky1 Received: 30 April 2020 / Accepted: 1 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The function of catalases A and T from the budding yeast Saccharomyces cerevisiae (ScCta1 and ScCtt1) is to decompose hydrogen peroxide (H2O2) to mitigate oxidative stress. Catalase orthologs are widely found in yeast, suggesting that scavenging H2O2 is crucial to avoid the oxidative damage caused by reactive oxygen species (ROS). However, the function of catalase orthologs has not yet been experimentally characterized in vivo. Here, we heterologously expressed Debaryomyces hansenii DhCTA1 and DhCTT1 genes, encoding ScCta1 and ScCtt1 orthologs, respectively, in a S. cerevisiae acatalasemic strain (cta1Δ ctt1Δ). We performed a physiological analysis evaluating growth, catalase activity, and H 2O2 tolerance of the strains grown with glucose or ethanol as carbon source, as well as under NaCl stress. We found that both genes complement the catalase function in S. cerevisiae. Particularly, the strain harboring DhCTT1 showed improved growth when ethanol was used as carbon source both in the absence or presence of salt stress. This phenotype is attributed to the high catalase activity of DhCtt1 detected at the exponential growth phase, which prevents intracellular ROS accumulation and confers oxidative stress resistance. Keywords Debaryomyces hansenii · Catalases · Heterologous yeast expression · Oxidative stress · Salt stress
Introduction Oxidative stress in yeast generally belongs to internal metabolic processes associated with respiration, but can also be generated through environmental stress conditions, such as salt stress, ethanol stress, among others [1–4]. High salinity (NaCl) disturbs yeast redox homeostasis resulting in oxidative damage [5]. High ethanol concentration affects Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02237-3) contains supplementary material, which is available to authorized users. * Claudia Segal‑Kischinevzky [email protected] 1
Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Cd. Universitaria, 04510 Coyoacán, Ciudad de México, México
Departamento de Bioquímica, Facultad de Medicina, Facultad de Medicina, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Cd. Universitaria, 04510 Coyoacán, Ciudad de México, México
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membrane permeability and stability inducing oxidative stress and, as a consequence, yeast viability and growth are inhibited during alcoholic fermentation [6–9]. Debaryomyces hansenii is a halotolerant yeast with the ability to tolerate diverse types of stress s
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