Topics in Fluorescence Spectroscopy DNA Technology

During the past 15 years, there has been remarkable progress in the analysis and manipulation of DNA and its use in nanotechnology. DNA analysis is ubiquitous in molecular biology, medical diagnostics, and forensics. Much of the readout technology is base

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Topics in Fluorescence Spectroscopy Edited by JOSEPH R. LAKOWICZ Volume 1: Techniques Volume 2: Principles Volume 3: Biochemical Applications Volume 4: Probe Design and Chemical Sensing Volume 5: Nonlinear and Two-Photon-Induced Fluorescence Volume 6: Protein Fluorescence Volume 7: DNA Technology

Topics in Fluorescence Spectroscopy Volume 7 DNA Technology

Edited by

JOSEPH R. LAKOWICZ Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology University of Maryland School of Medicine Baltimore, Maryland

KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW

eBook ISBN: Print ISBN:

0-306-47947-8 0-306-47387-9

©2005 Springer Science + Business Media, Inc. Print ©2003 Kluwer Academic/Plenum Publishers New York All rights reserved No part of this eBook may be reproduced or transmitted in any form or by any means, electronic, mechanical, recording, or otherwise, without written consent from the Publisher Created in the United States of America

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Contributors W. Patrick Ambrose Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Current address: Lawrence Livermore National Laboratory, Livermore, California 94551 Hong Cai Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Sabato D’Auria Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology, University of Maryland Medical School, Baltimore, Maryland 21201; and Institute of Protein Biochemistry and Enzymology, National Research Council, Naples, Italy 801215 Michael J. Difilippantonio Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-8010 Alexander N. Glazer Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200 Peter M. Goodwin Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 W. Kevin Grace Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Ignacy Gryczynski Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology, University of Maryland Medical School, Baltimore, Maryland 21201 Zygmunt Gryczynski Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology, University of Maryland Medical School, Baltimore, Maryland 21201 Robert C. Habbersett Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Mary E. Hawkins Pharmacology and Experimental Therapeutics Section, National Cancer Institute, Bethesda, Maryland 20892 v

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Contributors

Su-Chun Hung Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200 James H. Jett Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Richard A. Keller Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 8