Towards Low Cost Disposable High Throughput Screening Devices
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Towards low cost disposable high throughput screening devices Gerardo A. Diaz-Quijada1, Regis Peytavi2, André Nantel3, Emmanuel Roy1, Michel G. Bergeron2, Michel M. Dumoulin1 and Teodor Veres1. 1 Industrial Materials Institute, National Research Council of Canada, 75 de Mortagne, Boucherville, QC, J4B 6Y4, Canada. 2 Centre de recherche en infectiologie, Université Laval, 2705 Boul. Laurier, Sainte Foy, QC, G1V 4G2, Canada. 3 Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave., Montreal, QC H4P 2R2, Canada. ABSTRACT Microarrays have become one of the most convenient tools for high throughput screening, supporting major advances in genomics and proteomics. Other important applications can be found in medical diagnostics, detection of biothreats, drug discovery, etc. Integration of microarrays with microfluidic devices can be highly advantageous in terms of portability, shorter analysis time and lower consumption of expensive biological analytes. Since fabrication of microfluidic devices using traditional materials such as glass is rather expensive, there is a high interest in employing polymeric materials as a low cost alternative that is suitable for mass production. A number of commercially available plastic materials were reviewed for this purpose and poly(methylmethacrylate) and ZeonorTM 1060R were identified as promising candidates, for which methods for surface modification and covalent immobilization of DNA oligonucleotide were developed. In addition, we present proof-of-concept plastic-based microarrays with and without integration with microfluidics. INTRODUCTION Rapid progress in life sciences relies strongly on advancements in the areas of genomics and proteomics. Such advances are made possible with high throughput screening systems, which are for the most part based on microarray technologies. DNA microarrays, for example, play a vital role in the study of gene expression across the entire genome in a single experiment.1,2 Recently, there has been an increased interest in combining microfluidics with microarrays for potential applications in not only genomics and proteomics, but also in medical diagnostics, biothreat detection, drug discovery, chemical catalysis, etc. Medical diagnostics is an area of great interest and this has recently fueled an increased interest in the development of a disposable rapid medical theranostics (diagnostics and therapeutics) system for point-of-care use. We present herein our contribution to the selection of the plastic support, chemical functionalization and immobilization of biomolecules on plastic surfaces EXPERIMENTAL DETAILS All chemical reagents were purchased from Aldrich. Oligomers of DNA were custom synthesized by the microarraying facilities at NRC’s Biotechnology Research Institute (BRI). Optical transmission spectra were collected with a Beckman DU-640 spectrometer. Fluorescence
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spectra were acquired with a SPEX fluorolog2- F111AI using solid rectangular prisms (10 x 6 x 40 mm) for each pla
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