Towards next-generation model microorganism chassis for biomanufacturing
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MINI-REVIEW
Towards next-generation model microorganism chassis for biomanufacturing Yanfeng Liu 1,2 & Anqi Su 1,2 & Jianghua Li 1,2 & Rodrigo Ledesma-Amaro 3 & Peng Xu 4 & Guocheng Du 1,2 & Long Liu 1,2 Received: 27 July 2020 / Revised: 3 September 2020 / Accepted: 10 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Synthetic biology provides powerful tools and novel strategies for designing and modifying microorganisms to function as cell factories for biomanufacturing, which is a promising approach for realizing chemical production in a green and sustainable manner. Recent advances in genetic component design and genome engineering have enabled significant progresses in the field of synthetic biology chassis that have been developed for enzymes or biochemical production based on synthetic biology strategies, with particular reference to model microorganisms, such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, and Saccharomyces cerevisiae. In this review, strategies for engineering four different functional cellular modules which encompass the total process of biomanufacturing are discussed, including expanding the substrate spectrum for substrate uptake modules, refactoring biosynthetic pathways and dynamic regulation for product synthesis modules, balancing energy and redox modules, and cell membrane and cell wall engineering of product storage and secretion modules. Novel strategies of integrating and coordinating different cellular modules aided by synthetic co-culturing of multiple chassis, artificial intelligence– aided data mining for guiding strain development, and the process for designing automatic chassis development via biofoundry are expected to generate next generations of model microorganism chassis for more efficient biomanufacturing. Key points • Engineering of functional cellular modules facilitate next generations of chassis construction. • Global optimization of biosynthesis can be improved by metabolic models. • Data-driven and automatic strain development can improve microorganism chassis construction. Keywords Synthetic biology . Model microorganism chassis . Biomanufacturing . Cellular functional modules
Introduction Production of biochemicals via microorganism is a promising approach for manufacturing chemicals in a green and
* Long Liu [email protected] 1
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
2
Science Center for Future Foods, Jiangnan University, Wuxi 214122, China
3
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
4
Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA
sustainable manner with biomass as feedstock. This provides unique advantages, resulting in the efficient production of bioactive nutraceuticals and structurally complex natural products, as compared with chemical synthesis (Choi et al. 2019; Clomburg et al. 201
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