Transcriptome assembly of Modiolus modiolus and comparative analysis with Bathymodiolus platifrons
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Transcriptome assembly of Modiolus modiolus and comparative analysis with Bathymodiolus platifrons MENG Jie1, 2, 3, 4, YANG Mei5, 6, XU Fei1, 3, 4, LI Xinzheng5, 6, 7*, LI Li1, 2, 3, 4* 1 CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences (CAS),
Qingdao 266071, China 2 Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology,
Qingdao 266071, China 3 National and Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese
Academy of Sciences, Qingdao 266071, China 4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China 5 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 6 University of Chinese Academy of Sciences, Beijing 100039, China 7 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and
Technology, Qingdao 266071, China Received 18 August 2017; accepted 13 March 2018 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract
The genetic basis for bivalves’ adaptation and evolution is not well understood. Even few studies have focused on the mechanism of molluscan molecular evolution between the coastal intertidal zone and deep-sea environment. In our studies, we first conducted the transcritpome assembly of Modiolus modiolus mussels living in coastal intertidal zones. Also, we conducted transcriptome comparison analyses between M. modiolus and Bathymodiolus platifrons living in hydrothermal vents and cold methane/sulfide-hydrocarbon seeps. De novo assemblies of the clean reads yielded a total of 182 476 and 156 261 transcripts with N50 values of 1 769 and 1 545 in M. modiolus and B. platifrons. A total of 27 868 and 23 588 unigenes were identified, which also displayed the similar GO representation patterns. Among the 10 245 pairs of putative orthologs, we identified 26 protein-coding genes under strong positive selection (Ka/Ks>1) and 12 genes showing moderate positive selection (0.5
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