Transcriptome-Wide Identification and Expression Profiles of Masson Pine WRKY Transcription Factors in Response to Low P
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ORIGINAL ARTICLE
Transcriptome-Wide Identification and Expression Profiles of Masson Pine WRKY Transcription Factors in Response to Low Phosphorus Stress Fuhua Fan 1,2,3
&
Qingzhu Wang 3,4 & Huiping Li 4 & Guijie Ding 1,2 & Xiaopeng Wen 4
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Masson pine (Pinus massoniana Lamb.) is an economically important conifer tree that can be widely used for timber, pulp, and resin production. However, the phosphate (Pi) deficiency in tropical and subtropical forest soils poses severe challenges for the productivity of masson pine. WRKY transcription factors (TFs) have been proven to play important roles in plant responses to biotic and abiotic stresses, including low Pi stress; however, little is known about their roles in masson pine. To understand the roles of P. massoniana WRKY (PmWRKY) in low Pi stress, 25 putative WRKY TFs with complete WRKY domain from transcriptome sequencing data were identified. Based on their conserved domains and zinc-finger motif, the P. massoniana WRKY were divided into three groups. Structural feature analysis shows that PmWRKY proteins contain WRKYGQK/GKK/GRK domains and a C2H2/C2HC-type zincfinger structure. To putatively identify the function of PmWRKY members involved in low Pi stress, transcriptional profiles of 17 PmWRKYs in masson pine under different Pi stresses were systematically established using real-time quantitative RT-PCR analysis. Analysis demonstrates that the candidate PmWRKYs were involved in responses to Pi starvation—for example, PmWRKY11, 12, and 13 were upregulated both in P1 (Phosphorus content of 0.01 mM) and P2 (Phosphorus content of 0.06 mM) stresses. The available evidence conclusively sheds light on protein structures, evolutionary relationships, and expression patterns of WRKYs in response to low Pi stress of masson pine, which facilitates further functional identification and molecular breeding for the enhancement of lowphosphorous tolerance in this species. Keywords Masson pine . WRKY transcription factors . Phylogenic analysis . Differential expression . Low Pi stresses Fuhua Fan and Qingzhu Wang contributed equally to this work. Key Message To understand P. massoniana WRKY (PmWRKY) roles in low Pi stress, we performed a transcriptome-wide identification of WRKY TFs in masson pine. Judging from their conserved domains and zinc-finger motif, they were diversely homologous to those from Arabidopsis thaliana and Oryza sativa, and we divided them into three groups. Structural feature analysis shows that the PmWRKY proteins contained WRKYGQK/GKK/GRK domains and a C2H2/C2HC-type zinc-finger structure. Transcriptional profiles of 17 PmWRKYs in masson pine upon different Pi stresses were systematically established using real-time quantitative RT-PCR analysis, and candidate PmWRKYs involved in responses to Pi-deficiency were identified. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-020-01222-1) contains supplementary material, which is ava
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