Over-Expression of Masson Pine PmPT1 Gene in Transgenic Tobacco Confers Tolerance Enhancement to Pi Deficiency by Amelio
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ORIGINAL PAPER
Over-Expression of Masson Pine PmPT1 Gene in Transgenic Tobacco Confers Tolerance Enhancement to Pi Deficiency by Ameliorating P Level and the Antioxidants Ting Zhang 1,2 & Yi Hong 1 & XiaoPeng Wen 1
# Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Previously, we cloned the full sequence of masson pine (Pinus massoniana) phosphate transporter gene (PmPT1) from a phosphorus (Pi) deficiency tolerant strain. To further verify whether PmPT1 presumably function in angiosperms, i.e. tobacco, as well as to generate the new germplasm with high tolerance to Pi deficiency, currently, this gene was transferred into tobacco (Nicotiana tabacum) through Agrobacterium-mediated method. PmPT1 chiefly expressed in the roots of the transgenic plants, and considerably promoted the expression of two endogenous phosphate transporter genes of tobacco (NtPT1 and NtPT2) irrespectively of Pi status. Under low Pi conditions, the total P contents of the roots and shoots increased by 33.3% and 25.5%, respectively in L7, and by 30.7% and 23.9%, respectively in L18 in comparison with those of the wild type (WT). Also, the inorganic phosphorus (Pi) content of whole plants in L7 and L18 increased by 42.9% and 42.3%, respectively compared to the WT. The dry weight, contents of chlorophyll, soluble sugar and soluble protein, as well as the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were significantly elevated, conversely the MDA accumulation was obviously decreased in transgenic lines compared to the WT. Therefore, PmPT1, a phosphate transporter gene of Pht1 family from masson pine might function in tobacco and two overexpressed-PmPT1 transgenic lines substantially enhanced the tolerance of the transgenic tobacco to low-P stress, which was at least chiefly ascribed to the improvement of P accumulation and oxidant alleviation. Keywords PmPT1 . Genetic transformation . Tobacco . Low-phosphorus stress . Antioxidant Key message A masson pine phosphate transporter gene (PmPT1) was further transferred into tobacco using the Agrobacterium-mediated method. The transgenic plantlets were able to exhibit confers tolerance enhancement to Pi deficiency, which was at least chiefly ascribed to the improvement of expression of phosphate transporter genes, P accumulation and oxidant alleviation. * XiaoPeng Wen [email protected] Ting Zhang [email protected] Yi Hong [email protected] 1
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute for Forest Resources & Environment of Guizhou, Institute of Agro-Bioengineering/College of Life Sciences, Guizhou University, Guiyang 550025 Guizhou Province China
2
Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005 Guizhou Province China
Introduction Phosphorus is an essential nutrient for plant growth and development, and of high importanc
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