Proteomic Profiling Methods and Protocols
This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation, and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe
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Anton Posch Editor
Proteomic Profiling Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Proteomic Profiling Methods and Protocols
Edited by
Anton Posch Bio-Rad Laboratories GmbH, Munich, Germany
Editor Anton Posch Bio-Rad Laboratories GmbH Munich, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2549-0 ISBN 978-1-4939-2550-6 (eBook) DOI 10.1007/978-1-4939-2550-6 Library of Congress Control Number: 2015933382 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Sample Preparation Methods and Protein Techniques for Proteomic Profiling This volume is a comprehensive continuation and extension of a book called “2D PAGE: Sample Preparation and Fractionation” which was published in 2008. This book presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Individual technologies of each pillar combined into complementary and robust workflows render proteomic analysis of complex biological samples even more powerful and are the prerequisite to gain maximum value from biological samples in a single experiment. In this volume, basic but important sample preparation protocols are described again, followed by sophisticated procedures to enrich for specific protein classes and completed by the detailed description of integrated workflows for comprehensive protein analysis and characterization. The authors of the individual chapters are well-known protein biochemists, and all of them have set value to p
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