Twenty-four microsatellite markers for the gray mouse opossum ( Tlacuatzin canescens ): development from illumina paired
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TECHNICAL NOTE
Twenty-four microsatellite markers for the gray mouse opossum (Tlacuatzin canescens): development from illumina paired-end sequences Je´sica Arcangeli • Fernando A. Cervantes Stacey L. Lance • Ma. Isabel Salazar • Jorge Ortega
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Received: 19 October 2012 / Accepted: 29 October 2012 Ó Springer Science+Business Media Dordrecht 2012
Abstract Tlacuatzin canescens is an opossum species endemic to Mexico from southern Sonora to Oaxaca, with populations in Tres Marı´as Islands and in central Peninsula of Yucatan. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Twenty-four polymorphic (tri- and tetranucleotide) microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 8). J. Arcangeli F. A. Cervantes Departamento de Zoologia, Instituto de Biologı´a, Universidad Nacional Auto´noma de Me´xico, Apartado Postal 70-153, 04510 Mexico, Distrito Federal, Mexico J. Arcangeli Posgrado en Ciencias Biolo´gicas, Universidad Nacional Auto´noma de Me´xico, Av. Universidad 3000, 04510 Mexico, Distrito Federal, Mexico S. L. Lance Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29803, USA Ma. I. Salazar Laboratorio de Inmunologı´a Celular e Inmunopatoge´nesis, Departamento de Inmunologı´a, Escuela Nacional de Ciencias Biolo´gicas, Instituto Polite´cnico Nacional, Prolongacio´n de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico, Distrito Federal, Mexico J. Ortega (&) Laboratorio de Ictiologı´a y Limnologı´a, Posgrado en Ciencias Quimicobiolo´gicas, Departamento de Zoologı´a, Escuela Nacional de Ciencias Biolo´gicas, Instituto Polite´cnico Nacional, Prolongacio´n de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico, Distrito Federal, Mexico e-mail: [email protected]
Levels of expected heterozygosity across all markers was fairly low (mean HE = 0.477, mean HO = 0.480). Keywords Illumina PAL_FINDER Microsatellites Tlacuatzin canescens
The gray mouse opossum (Tlacuatzin canescens) is an endemic Mexican marsupial (Ramı´rez-Pulido et al. 2005), occurring in seasonally dry tropical forest along the Pacific versant from south Sonora to Chiapas; through the Balsas river basin, with some isolated populations occurring in the Peninsula of Yucata´n and in Tres Marı´as Islands (Voss and Jansa 2003). Its dorsal fur is gray or brownish gray with a black mask around eyes and color white or cream in ventral area. The ears are broad, rounded and naked; its tail is prehensile, naked except in the base and faintly bicolor; pouch is absent in females (Zarza et al. 2003). Referring to its taxonomic status, after a combined analysis with molecular and morphological data, authors concluded that Marmosa canescens diverged from other species of the genera, and was nominated Tlacuatzin, the na´huatl word for ‘‘opossum’’ (Voss
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