UHPLC-QTOFMS-based metabolomic analysis of serum and urine in rats treated with musalais containing varying ethyl carbam
- PDF / 3,600,732 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 18 Downloads / 146 Views
RESEARCH PAPER
UHPLC-QTOFMS-based metabolomic analysis of serum and urine in rats treated with musalais containing varying ethyl carbamate content Weihua Wang 1 & ZhanJiang Han 1 & Dongqi Guo 1 & Yanju Xiang 1 Received: 6 July 2020 / Revised: 7 August 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The aim of this work is to investigate the effect of the ethyl carbamate (EC) content in musalais on the metabolism of rats. Electron beam irradiation was performed to decrease the content of EC in musalais, and Sprague Dawley rats were subjected to intragastric administration of musalais with varying EC content (high, medium, and low groups). Control rats were fed normally without any treatment. Serum and urine samples were analyzed using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Principal component analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) were performed to detect changes in the metabolite profile in the serum and urine in order to identify the differential metabolites and metabolic pathways. The results demonstrated clear differences in the serum and urine metabolic patterns between control and treatment groups. Ions in treatment groups with variable importance in the projection of >1 (selected from the OPLS-DA loading plots) and Ps < 0.05 (Student t test) compared to control group were identified as candidate metabolites. Analysis of the metabolic pathways relevant to the identified differential metabolites revealed that high EC content in musalais (10 mg/kg) mainly affected rats through valine, leucine, and isoleucine biosynthesis and nicotinate and nicotinamide metabolism, which were associated with energy metabolism. In addition, this work suggests that EC can induce oxidative stress via inhibition of glycine content. Keywords Musalais . Ethyl carbamate . UHPLC-QTOFMS . Metabolomics . Multivariate data analysis
Introduction Musalais is a traditional wine made with local grapes planted by the Uygur people, a minority in southern Xinjiang, China. With a history of more than 2000 years, musalais is an important contributor to the local culture and tourist economy [1]. Musalais is brewed by traditional fermentation processes, during which numerous substances containing procyanidin are produced that are beneficial to human health [2]. In addition, ethyl carbamate (EC) is produced during the fermentation Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02900-5) contains supplementary material, which is available to authorized users. * ZhanJiang Han [email protected] 1
College of Life Science, Tarim University, Alaer, Xinjiang 843300, China
process. EC is commonly found in various fermented alcoholic beverages and food products including wine, cheese, bread, whisky, and yogurt [3]. It has been used as a co-solvent for sedative drugs and water-insoluble analgesics for many years in Japan to dissolve organic mater
Data Loading...
