Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet
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Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet* V. B. Vays1, I. M. Vangeli1, O. A. Averina1, M. L. Lovat2, and L. E. Bakeeva1,a** 1
Belozersky Institute of PhysicoChemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia 2 Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia a email: [email protected] Received July 7, 2020 Revised July 7, 2020 Accepted July 18, 2020
Abstract—The significant destructive changes in ultrastructure of hepatocytes from laboratory mice kept in different vivari ums in Moscow and fed with dry laboratory animal diets acquired from different domestic manufacturers that were not stan dardized for initial products were demonstrated using electron microscopy. Furthermore, disruption in the ultrastructure of liver parenchymal cells occurred regardless of the animal status (SPF or conventional), conditions of various vivariums, as well as the feed manufacturer. At the same time, studies on ultrastructure of liver hepatocytes from mice kept in the Charles River Laboratory facilities in Germany and fed with the Altromin Spezialfutter laboratory animal diet (GmbH & Co., Germany) that was produced using quality control of ingredients did not reveal destructive changes in the internal ultra structure of hepatocytes. However, if these mice were later fed with the food produced in local manufactures, changes in the structure of liver cells developed after 2 months. Thus, feeding with dry diet from the domestic producers of an unspecified composition causes significant changes in the ultrastructure of hepatocytes in control animals, reflecting the development of some pathological processes in the body. DOI: 10.1134/S0006297920090084 Keywords: mitochondria, hepatocytes, ultrastructure, mice, standard laboratory diet
INTRODUCTION Liver is the largest gland of the digestive system, which performs a wide variety of very important func tions. Liver tissues were the first widely studied by elec tron microscopy. Rapid progress of this method resulted in accumulation of a huge amount of data on the ultra fine structure of the liver tissue. The ultrastructure of hepatocytes was studied in detail in some classic works in the 19501960s. For the first time the ultrastructure of the liver cells was described by Dalton et al. [1] and Fawcett et al. [2]. However, the work of Bruni and Porter [3] who
Abbreviations: agEPR, agranular EPR; EPR, endoplasmic reticulum; grEPR, granular EPR; IVC, individually ventilated cage; NAFLD, nonalcoholic fatty liver disease; SPF status, specificpathogenfree animals; VEC, Complex of the Animal Facilities and the Research Unit Vivarium, Institute of Mitoengineering of Lomonosov Moscow State University. * The article is presented by the EditorinChief academician V. P. Skulachev. ** To whom correspondence should be addressed.
examined features of the hepatocyte ultrastructure using different techniques for liver tissue fixation has been con sidered classic and remains the world s
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