Various cross-linking methods inhibit the collagenase I degradation of rabbit scleral tissue
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RESEARCH ARTICLE
Open Access
Various cross-linking methods inhibit the collagenase I degradation of rabbit scleral tissue Konstantin Krasselt1, Cornelius Frommelt1, Robert Brunner2, Franziska Georgia Rauscher3, Mike Francke1,3* Nicole Körber1
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Abstract Background: Collagen cross-linking of the sclera is a promising approach to strengthen scleral rigidity and thus to inhibit eye growth in progressive myopia. Additionally, cross-linking might inhibit degrading processes in idiopathic melting or in ocular inflammatory diseases of the sclera. Different cross-linking treatments were tested to increase resistance to enzymatic degradation of the rabbit sclera. Methods: Scleral patches from rabbit eyes were cross-linked using paraformaldehyde, glutaraldehyde or riboflavin combined with UV-A-light or with blue light. The patches were incubated with collagenase I (MMP1) for various durations up to 24 h to elucidate differences in scleral resistance to enzymatic degradation. Degraded protein components in the supernatant were detected and quantified using measurements of Fluoraldehyde oPhthaldialdehyde (OPA) fluorescence. Results: All cross-linking methods reduced the enzymatic degradation of rabbit scleral tissue by MMP1. Incubation with glutaraldehyde (1%) and paraformaldehyde (4%) caused nearly a complete inhibition of enzymatic degradation (down to 7% ± 2.8 of digested protein compared to control). Cross-linking with riboflavin/UV-A-light reduced the degradation by MMP1 to 62% ± 12.7 after 24 h. Cross-linking with riboflavin/blue light reduced the degradation by MMP1 to 77% ± 13.5 after 24 h. No significant differences could be detected comparing different light intensities, light exposure times or riboflavin concentrations. Conclusions: The application of all cross-linking methods increased the resistance of rabbit scleral tissue to MMP1degradation. Especially, gentle cross-linking with riboflavin and UV-A or blue light might be a clinical approach in future. Keywords: Scleral cross-linking, Rabbit sclera, Glutaraldehyde, Paraformaldehyde, Riboflavin/UV-A-light, Riboflavin/blue light
* Correspondence: [email protected] 1 Paul-Flechsig-Institute of Brain Research, Leipzig University, Liebigstraße 19, 04103 Leipzig, Germany 3 Institute for Medical Informatics, Statistics and Epidemiology (IMISE), Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the art
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