West Nile Virus Methods and Protocols
This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination o
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Tonya M. Colpitts Editor
West Nile Virus Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
West Nile Virus Methods and Protocols
Edited by
Tonya M. Colpitts Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA
Editor Tonya M. Colpitts Department of Pathology, Microbiology and Immunology University of South Carolina School of Medicine Columbia, SC, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3668-7 ISBN 978-1-4939-3670-0 (eBook) DOI 10.1007/978-1-4939-3670-0 Library of Congress Control Number: 2016940951 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Protocols for West Nile Virus Research is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile virus (WNV). The target audience is comprised of researchers such as virologists, molecular biologists, microbiologists, and other scientists working with WNV. The methods provided here will serve as a comprehensive guide for those conducting research in the field of WNV biology, including life cycle, pathogenesis, impact of infection, and possible treatment/prevention development. A brief introduction along with a discussion of WNV laboratory safety is also included here. Columbia, SC, USA
Tonya M. Colpitts
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Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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