Regenerative Hepatic Effect of Red Propolis Extract Administration After Partial Hepatectomy in Rats
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ORIGINAL ARTICLE
Regenerative Hepatic Effect of Red Propolis Extract Administration After Partial Hepatectomy in Rats Ana Karina Rocha Hora Mendonça 1 & Carla Viviane Freitas de Jesus 1 & Felipe Mendes de Andrade de Carvalho 1 Yasmim Anayr Costa Ferrari 1 & Maria Julia Nardelli 1 & Sydney Correia Leão 1 & Sonia Oliveira Lima 1
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Received: 29 June 2020 / Accepted: 7 September 2020 / Published online: 17 September 2020 # Sociedade Brasileira de Farmacognosia 2020
Abstract Human liver proliferation resulting from partial hepatectomy or from acute harmful stimuli may lead to liver failure. The aim of the present study was to investigate the acute effect of hydroethanolic extract of red propolis on the proliferation of hepatocytes after enlarged hepatectomy. Propolis is a resinous product made by honeybees with wide therapeutic application. The following doses were used in the treatment with hydroethanolic extract: 3, 10, and 30 mg/kg/day; it was administered via gavage, once a day; and propolis delivery started 3 days before the 70% hepatectomy procedure. Blood and liver samples were collected 24 and 48 h after liver resection. Serum aminotransferase measurements were taken, and histopathological and immunohistochemical analyses were carried out to find the antigen marker of Ki-67 cell proliferation. Daidzein, formononetin, and biochanin A were the chemical compounds identified in the hydroethanolic extract of red propolis. Enlarged hepatectomy led to mild inflammatory reaction, cytoplasmic vacuolization, microgoticular steatosis, mild sinusoidal congestion, and manifestation of rare apoptotic bodies in the remaining liver tissue. The extracellular matrix showed preserved reticular weave and deposition of some collagen fibers. The treatment based on 3 mg/kg of hydroethanolic extract of red propolis significantly increased the hepatocellular proliferation rate in comparison with the control. These results suggest that the pre-treatment with hydroethanolic extract of red propolis has stimulated hepatocellular proliferation during the acute phase of the hepatic regenerative process. Keywords Hepatocellular proliferation . Hepatectomy . Immunohistochemistry . Liver regeneration . Propolis
Introduction Partial hepatectomy is a common, safe, and viable therapeutic technique developed in the last two decades by liver surgeons in order to enable liver cancer excision and to collect liver parenchyma from living donors during liver transplantation. Chronic, progressive, and irreversible liver diseases as well as acute liver failure at terminal stage caused by chemical or viral damage are factors adopted for liver transplantation
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s43450-020-00101-w) contains supplementary material, which is available to authorized users. * Ana Karina Rocha Hora Mendonça [email protected] 1
Instituto de Tecnologia e Pesquisa, Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil
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