A functional molecular marker for detecting blister blight disease resistance in tea ( Camellia sinensis L.)
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ORIGINAL ARTICLE
A functional molecular marker for detecting blister blight disease resistance in tea (Camellia sinensis L.) K. H. T. Karunarathna1 · K. M. Mewan2 · O. V. D. S. J. Weerasena1 · S. A. C. N. Perera3 · E. N. U. Edirisinghe4 Received: 15 May 2020 / Accepted: 10 November 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Identification of an EST-SSR molecular marker associated with Blister blight, a common fungal disease of tea, facilitating marker-assisted selection, marking a milestone in tea molecular breeding. Abstract lister blight (BB) leaf disease of tea, caused by the fungus Exobasidium vexans, results in 25–30% crop loss annually. BB is presently controlled by Cu based fungicides, but genetic resistance is the most viable option in disease management. Tea is a naturally out-crossing, woody perennial necessitating a long time for completion of a breeding programme. Marker-assisted selection (MAS) is vital to expedite breeding programmes and also for better accuracy in gene identification. The aim of the current research was to derive marker-trait associations using an F1 population segregating for BB. The population was genotyped at 11 expressed sequence tag simple sequence repeat loci followed by detecting the alleles by fragment analysis. The genotypic and phenotypic data were subjected to single-marker analysis resulting in the identification of EST-SSR073 as a diagnostic marker amplifying three alleles of the sizes, 168, 170 and 190 bp in F 1. Of them, alleles 190 and 168 bp were confirmed to concur BB resistance and susceptibility, respectively. The alleles were validated in a panel of 64 tea cultivars, resulting in the amplification of 12 alleles at EST-SSR073. The EST-SSR073 allele sequences matched with Camellia sinensis photosystem-I reaction center subunit-II. The marker EST-SSR073 can be effectively used in breeding tea against BB, recording a milestone in MAS in tea. Keywords Biotic stress resistance · EST-SSR marker · Exobasidium fungi · Marker assisted selection · Molecular breeding
Introduction
Communicated by Howard S. Judelson. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02637-6) contains supplementary material, which is available to authorized users. * S. A. C. N. Perera [email protected] 1
Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka
2
Department of Biotechnology, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Kuliyapitiya, Sri Lanka
3
Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri Lanka
4
Biochemistry Division, Tea Research Institute of Sri Lanka, Talawakelle, Sri Lanka
Tea, Camellia sinensis (L.) O. Kuntze, (family-Theaceae) is a pre-dominantly out-breeding woody perennial (2n = 30). The economically important part of the tea, the green leaves are manufactured to one of the most po
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