Effects of shading on lignin biosynthesis in the leaf of tea plant ( Camellia sinensis (L.) O. Kuntze)

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ORIGINAL ARTICLE

Effects of shading on lignin biosynthesis in the leaf of tea plant (Camellia sinensis (L.) O. Kuntze) Rui‑Min Teng1 · Yong‑Xin Wang1 · Hui Li1 · Shi‑Jia Lin1 · Hao Liu1 · Jing Zhuang1  Received: 15 June 2020 / Accepted: 12 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020

Abstract Shading can effectively reduce photoinhibition and improve the quality of tea. Lignin is one of the most important secondary metabolites that play vital functions in plant growth and development. However, little is known about the relationship between shading and xylogenesis in tea plant. To investigate the effects of shading on lignin accumulation in tea plants, ‘Longjing 43’ was treated with no shading (S0), 40% (S1) and 80% (S2) shading treatments, respectively. The leaf area and lignin content of tea plant leaves decreased under shading treatments (especially S2). The anatomical characteristics showed that lignin is mainly distributed in the xylem of tea leaves. Promoter analysis indicated that the genes involved in lignin pathway contain several light recognition elements. The transcript abundances of 12 lignin-associated genes were altered under shading treatments. Correlation analysis indicated that most genes showed strong positive correlation with lignin content, and CsPAL, Cs4CL, CsF5H, and CsLAC exhibited significant positively correlation under 40% and 80% shading treatments. The results showed that shading may have an important effect on lignin accumulation in tea leaves. This work will potentially helpful to understand the regulation mechanism of lignin pathway under shading treatment, and provide reference for reducing lignin content and improving tea quality through shading treatment in field operation. Keywords  Lignin shading · Leaf development · Anatomical structure · Gene expression · Camellia sinensis

Introduction Light is an important factor that regulates plant growth and development. The light radiation could affect plant morphogenesis, photosynthesis, and phototropism (Monteith 1965; Chory 1997), which established itself as the driving force of the photosynthetic processes that convert carbon dioxide into organic products (Monteiro et al. 2014). The physiological and molecular basis of light-induced process has been well elaborated in higher plant (Castillon et al. 2007). The mechanism of light radiation regulates phenylpropanoid biosynthesis has been reported in some model plants (Li et al. 1993; Communicated by Stefan Hohmann. Electronic supplementary material  The online version of this article (https​://doi.org/10.1007/s0043​8-020-01737​-y) contains supplementary material, which is available to authorized users. * Jing Zhuang [email protected] 1



Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Albert et al. 2009; Matus et al. 2009; Robin et al. 2009). Many metabolites are derived from downstream branches of the phenylpropanoid pathway, including lignin. Lignin is a widely exist phenolic biopolym