A novel mutation in TFL1 homolog sustaining determinate growth in cucumber ( Cucumis sativus L.)
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ORIGINAL ARTICLE
A novel mutation in TFL1 homolog sustaining determinate growth in cucumber (Cucumis sativus L.) Martin Kagiki Njogu1,2 · Fan Yang1 · Ji Li1 · Xueyan Wang1 · Joshua Otieno Ogweno3 · Jinfeng Chen1 Received: 16 May 2020 / Accepted: 11 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message BSA-seq combined with whole-genome resequencing map-based cloning delimited the cucumber detnovel locus into a 44.5 kb region in chromosome 6 harboring a putative candidate gene encoding a phosphatidylethanolamine-binding protein (CsCEN). Abstract Determinate and indeterminate growth habits of cucumber can affect plant architecture and crop yield. The TERMINAL FLOWER 1 (TFL1) controls determinate/indeterminate growth in Arabidopsis. In this study, a novel mutation in cucumber TFL1 homolog (CsCEN) has shown to regulate determinate growth and product of terminal flowers in cucumber (Cucumis sativus L.), which is similar to the function of CsTFL1 as previously reported. Genetic analysis in two determinate genotypes (D226 and D082) and indeterminate genotype (CCMC) revealed that a single recessive gene is responsible for this determinate growth trait. With the combination of BSA-seq and whole-genome resequencing, the locus of determinate-novel (det-novel) trait was mapped to a 44.5 kb genomic region in chromosome 6. Sequence alignment identified one non-synonymous SNP mutation (A to T) in the third exon of CsCEN, resulting in an amino acid substitution (Thr to Pro), suggesting that determinate growth might be controlled by a novel gene CsCEN (Csa6G152360) which differed from the reported CsTFL1 gene. The CsCEN expression level in shoot apexes and axillary buds was significantly lower in D226 compared to CCMC, suggesting its essential role in sustaining indeterminate growth habit. Identification and characterization of the CsCEN in the present study provide a new insight into plant architecture modification and development of cucumber cultivars suited to mechanized production system.
Introduction
Martin Kagiki Njogu, Fan Yang and Ji Li have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03671-4) contains supplementary material, which is available to authorized users.
Cucumber (Cucumis sativus L.) is an economically important vegetable crop widely cultivated in the world. Most cultivated cucumber have indeterminate growth habit which is characterized by tall plant which continuously produce inflorescences, nodes and branches, while stems have prominent nodes and internodes. Indeterminate growth habit brings about sustainable fruit production from different parts of the
* Joshua Otieno Ogweno [email protected]
Xueyan Wang [email protected]
* Jinfeng Chen [email protected]
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Martin Kagiki Njogu [email protected]
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
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