A novel narnavirus from the plant-pathogenic fungus Magnaporthe oryzae
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A novel narnavirus from the plant‑pathogenic fungus Magnaporthe oryzae Yuan Lin1 · Jia Zhou1,2 · Xuan Zhou1 · Simin Shuai1 · Rendi Zhou1 · Hongliu An1,3 · Shouguo Fang1,3 · Songbai Zhang1,3 · Qingchao Deng1,3 Received: 6 October 2019 / Accepted: 8 February 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel mycovirus with the proposed name “Magnaporthe oryzae narnavirus virus 1” (MoNV1), was described in the rice blast fungus Magnaporthe oryzae. The virus has a single-stranded (+ss) RNA genome of 2452 nucleotides, contains a single open reading frame (ORF) predicted to encode an RNA-dependent RNA polymerase (RDRP), and is closely related to some viruses of the genus Narnavirus, family Narnaviridae, including Aspergillus fumigatus narnavirus 1 (AfNV1), Neofusicoccum parvum narnavirus 2 (NpNV2) and Alternaria tenuissima narnavirus 1 (AtNV2). Genome sequence comparisons and phylogenetic analysis suggested that MoNV1 is a new member of the genus Narnavirus. The RDRPs of MoNV1 and some closely related narnaviruses do not contain a typical metal-binding “GDD” motif and catalytic site. Further studies are needed to investigate the replication mechanism of these viruses. Mycoviruses with increasingly diverse genomes have been described in all major fungal groups [3, 17, 19, 25]. Although most mycoviruses have double-stranded (ds) or positive-sense single-stranded (+ss) RNA genomes, mycoviruses with a negative-sense single-stranded (−ss) RNA genome or a DNA genome also have been identified [11, 27]. Since some mycoviruses can reduce the severity of fungal plant diseases, the biological control potential of mycovirus is attracting more and more attention [3, 17, 19, 25]. Handling Editor: Ioly Kotta-Loizou. Yuan Lin and Jia Zhou contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04586-7) contains supplementary material, which is available to authorized users. * Songbai Zhang [email protected] * Qingchao Deng [email protected] 1
College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
2
College of Plant Protection, Hainan University, Haikou 570228, Hainan, China
3
Hubei Engineering Research Center for Pest Forewarning and Management, Jingzhou 434025, Hubei, China
Magnaporthe oryzae, the causal agent of rice blast, is the most destructive pathogen of rice and causes enormous yield losses worldwide every year. A number of mycoviruses have been reported to infect M. oryzae, including dsRNA viruses such as Magnaporthe oryzae viruses 1, 2 and 3 (MoV1, MoV2 and MoV3) of the family Totiviridae [13, 22, 26], Magnaporthe oryzae partitivirus 1 (MoPV1) of the family Partitiviridae [2], and Magnaporthe oryzae chrysovirus 1 (MoCV1) of the family Chrysoviridae [4, 23, 24]; (+ss) RNA viruses such as Magnaporthe oryzae ourmia-like virus 1 (MOLV1) and Magnaporthe oryzae ourmia-like virus 4 (MOLV4) of the family Botourmiaviridae [6, 10], and Magnaport
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