Studies on interaction and illumination damage of CS-Fe 3 O 4 @ZnS:Mn to bovine serum albumin
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RESEARCH PAPER
Studies on interaction and illumination damage of CS-Fe3O4@ZnS:Mn to bovine serum albumin Li Liu • Ling Xiao • Hua-Yue Zhu • Xiao-Wen Shi
Received: 17 August 2012 / Accepted: 21 December 2012 Ó Springer Science+Business Media Dordrecht 2013
Abstract In this study, the interaction of chitosan (CS) coated CS-Fe3O4@ZnS:Mn magnetic-fluorescent nanoparticles (MFNPs) with bovine serum albumin (BSA) was studied by means of ultraviolet–visible and fluorescence spectra; evidences for the damage of BSA molecule in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination were also obtained. The results show that the dominating fluorescence quenching mechanism of CS-Fe3O4@ZnS:Mn MFNPs with BSA belongs to static quenching. Fluorescence resonance energy transfer (FRET) occurred from BSA to CS-Fe3O4@ZnS:Mn MFNPs. The thermodynamic parameters indicated that electrostatic interaction play major roles in CS-Fe3O4@ZnS:Mn-BSA, while binding processes exist spontaneously. In addition, the damage of CS-Fe3O4@ZnS:Mn MFNPs to BSA
L. Liu L. Xiao H.-Y. Zhu X.-W. Shi College of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and EnvironmentalBiotechnology Key Laboratory, Wuhan University, Wuhan 430072, People’s Republic of China e-mail: [email protected] H.-Y. Zhu e-mail: [email protected] X.-W. Shi e-mail: [email protected] L. Liu L. Xiao (&) H.-Y. Zhu X.-W. Shi The Luojia Mountain of Wuchang, Wuhan 430072, Hubei, People’s Republic of China e-mail: [email protected]
molecule under UV illumination was studied under various experimental parameters. It was proved that: the damage of BSA is prone to happen in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination, there is synergic effect of oxygen and UV illumination on the damage of BSA, and the fluorescence quenching of BSA by CS-Fe3O4@ZnS:Mn MFNPs under UV illumination is mainly a result of a photo-induced free radical procedure. Keywords Magnetic-fluorescent nanoparticles Bovine serum albumin (BSA) Interaction UV illumination Reactive oxygen species (ROS)
Introduction In recent years, magnetic-fluorescent nanoparticles (MFNPs) with both fluorescent and magnetic properties are getting increasing attentions, as MFNPs can be applied in broad fields including biomedicine, information storage, and so on (Yi et al. 2005; Sun et al. 2010; Xiao and Xiao 2009; Zhang et al. 2008). In particular, significant progresses in their biological applications such as dual modal optical/magnetic resonance imaging and drug delivery have attracted intense research interest (Zhang et al. 2011; Wang et al. 2010b). Magnetic-fluorescent nanoparticles composed of biocompatible quantum dots and magnetic nanoparticles have been examined as theranostics, the combination of diagnostics and therapies, providing a
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new concept in the battles with various major diseases such as cancer (Cheng et al. 2012). The fluorescence of quantum dots is intrinsic, bright, prolonged, and surpasses the sensitivity and resolution capability of
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