Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamer
- PDF / 921,230 Bytes
- 12 Pages / 547.087 x 737.008 pts Page_size
- 58 Downloads / 190 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL RESEARCH PAPER
Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamer Fatima Shatila . I˙hsan Yas¸ a . H. Tansel Yalc¸ın
Received: 3 April 2020 / Accepted: 19 May 2020 Ó Springer Nature B.V. 2020
Abstract The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maximum inhibitory effect equivalent to 24.34% and 26.81% was recorded when Apt17 was co-incubated with S. enteritidis TM 6 and S. enteritidis TM 68 respectively for 13 h. The inhibitory effect of Apt17 was also confirmed with Triphenyl Tetrazolium Chloride. Under Scanning Electron Microscope, the presence of Apt17 resulted in altered three dimensional structure. While the treated cells of S. enteritidis TM 6 were arranged as monolayers, the sessile aggregates of S. enteritidis TM 68 appeared thinner and exhibited less surface coverage when compared to control. Moreover, the treated cells lost their exopolysaccharide matrix. The co-incubation of Apt17 with ampicillin MIC/10 for 24 h, inhibited the biofilms of S. enteritidis TM 6 and S. enteritidis TM 68 by 12.5 and 20.9% respectively. This study demonstrated quantitative and qualitative antibiofilm effect F. Shatila (&) I˙. Yas¸ a H. T. Yalc¸ın (&) Basic and Industrial Microbiology Section, Biology Department, Faculty of Science, Ege University, 35100 Izmir, Turkey e-mail: [email protected] H. T. Yalc¸ın e-mail: [email protected]
of Apt17 against the biofilms of two Salmonella enteritidis strains. According to our knowledge, this is the first study employing an aptamer that targets SipA protein to inhibit biofilm formation in Salmonella. Keywords Salmonella enteritidis Biofilms Inhibition Salmonella invasion protein A Scanning electron microscope Aptamer
Introduction Biofilms are organized 3D sessile communities. The cells, which are embedded in a self-secreted exopolysaccharide matrix, demonstrate different morphological, physiological and metabolic activity from their planktonic counterparts (Lopes et al. 2011; Galie´ et al. 2018). Such characteristics provide biofilm cells with resistance against various stressful environmental conditions (Roy et al. 2018). Salmonella enterica serovars are among biofilm forming pathogens. Salmonella biofilms form on food processing surfaces as a result of inefficient hygiene practices and function as reservoirs that contaminate food surfaces causing foodborne outbreaks (Gonzales-Rivas et al. 2018). On the other hand, the formation of Salmonella biofilms on epithelial cells and human gallstones results in the asymptomatic carrier state, which in return causes chronic acute human infections (Gonzalez- Escobedo
123
Biotechnol Lett
and Gunn 2013; Gunn et al. 2014). The formation of these res
Data Loading...
