Analysis of the complete mitochondrial genome of the giant honeybee, Apis dorsata , (Hymenoptera: Apidae) in Thailand
- PDF / 901,124 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 109 Downloads / 262 Views
TECHNICAL NOTE
Analysis of the complete mitochondrial genome of the giant honeybee, Apis dorsata, (Hymenoptera: Apidae) in Thailand Jun‑ichi Takahashi1 · Sureerat Deowanish2 · Hisashi Okuyama1 Received: 15 November 2017 / Accepted: 25 November 2017 © Springer Science+Business Media B.V., part of Springer Nature 2017
Abstract The giant honeybee, Apis dorsata, is a species native to the East Asian continent and some islands of Sundaland and the Philippines. We analyzed, for the first time, the complete mitochondrial genome of A. dorsata from Thailand using next generation sequencing. The mitochondrial genome was found to be a circular molecule of 15,279 bp in length, which consisted of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 ribosomal RNA genes and an AT rich region. Start codons ATA, ATG and ATT were found in two, three and ten genes, respectively, while stop codons TAA and TAG were observed in 12 and 1 gene, respectively. All tRNA genes formed typical cloverleaf secondary structures, except for His (H) and Tyr (Y). The heavy (H)-strand was found to have nine PCGs and 14 tRNA genes, while the light (L)-strand was found to contain four protein-coding, eight tRNA, and two rRNA genes. Keywords Apis dorsata · Giant honeybee, migratory species · Thailand · tRNA structure The giant honeybee group (subgenus Megapis) is distributed in forests on the Southeast Asian continent and in partial regions of Sundaland and the Philippines (Sakagami et al. 1980; Ruttner 1988; Underwood1990; Otis 1996; Oldroyd and Wongsiri 2006). Phylogenetic analysis of the giant honeybees suggested the presence of two species, Apis dorsata and Apis laboriosa, as well as a possible third species, Apis breviligula, and the A. dorsata group may contain several subspecies or valid species (Sakagami et al. 1980; Ruttner 1988; Lo et al. 2010). Morphometric analyses between A. dorsata and A. laboriosa found differences in variables including size and cubital index (Cao et al. 2012). Woyke et al. (2012) also reported that the giant honeybees A. dorsata and A. laboriosa construct nests of different shapes Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12686-017-0942-7) contains supplementary material, which is available to authorized users. * Jun‑ichi Takahashi [email protected]‑su.ac.jp 1
Facluty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan
Center of Excellent in Entomology : Bee Biology, Biodiversity of insects and mites, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
2
and sizes. Molecular phylogenetic analyses of partial DNA sequences also indicated that A. dorsata and A. laboriosa are sister taxa, and the Philippine giant honeybee (A. breviligula) was also described as a separate species from A. dorsata (Lo et al. 2010; Cao et al. 2012). Apis dorsata inhabit forests from lowlands to mountainous regions that are widely distributed from India to southwestern China, and from Indonesia to Borneo Island (Ruttner 1988; Otis 1996; Oldroyd
Data Loading...
