The complete mitochondrial genome of Triplophysa brevibarba with phylogenetic consideration
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TECHNICAL NOTE
The complete mitochondrial genome of Triplophysa brevibarba with phylogenetic consideration Yanhua Zhang1 · Zhe Wang2 · Zhimin Zhang2 · Mingsong Wu2 · Jianying Chen3 · Yanyu Zhao2
Received: 2 May 2017 / Accepted: 8 May 2017 © Springer Science+Business Media Dordrecht 2017
Abstract Triplophysa brevibarba is distributed in the upstream of Ya-lung River. The conservation status of this fish has been assessed as near threatened due to overfishing and habitat destruction. In the present study, we determined the first complete mitochondrial genome of T. brevibarba. This genome was 16,570 bp in size and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a typical control region. The overall nucleotide composition was: 28.2% A, 28.5% T, 25.3% C, and 18.0% G, with a total A + T content of 56.7%. The genome size, gene arrangement, and composition were similar to those of other Triplophysa species. Phylogenetic analysis of all 42 Nemacheilidae species showed that Triplophysa is a monophyletic group and T. brevibarba was clustered with Triplophysa minxiannesis and Triplophysa robusta located in the basal clade of Triplophysa. Keywords Triplophysa brevibarba · Nemacheilidae · Mitochondrial genome · Tibetan Plateau Triplophysa brevibarba (Cypriniformes, Nemacheilidae), is an endemic fish of the Tibetan Plateau, which mainly distributes in the upper reaches of the Ya-lung River (Ding 1993). As one of the most species-rich groups in plateau * Yanyu Zhao [email protected] 1
College of Life Sciences, Jiangsu Normal University, Xuzhou, China
2
Department of Medical Genetics, Zunyi Medical University, Zunyi 563000, China
3
Department of Resource Environment, Qiannan Normal College for Nationalities, Duyun, China
waters, Triplophysa spp. play the significant role for fragile ecosystem of the plateau area. Recently, the marked human activities including construction of hydropower dams and overfishing resulted in the remarkable reduction of natural population size for endemic fishes (Ceballos et al. 2015). Additionally, the exotic species invasions accelerated the deterioration of ecosystem and species extinctions. The latest red list of China’s vertebrates showed that there were one extinct species (Triplophysa cakaensis), three critically endangered species (Triplophysa bombifrons, Triplophysa cuneicephala, and Triplophysa gejiuensis), two endangered species (Triplophysa lacustris and Triplophysa pappenheimi), and seventeen vulnerable and near threatened species (including T. brevibarba), accounting for about 18.8% of total number of Triplophysa species in China. Yet we know little knowledge about many of these Triplophysa species, and over half of Triplophysa spp. do not have any molecular data. Mitochondrial DNA has been widely used as a powerful molecular marker for species identification, phylogenetic analysis, population structure and dynamic studies (Cameron 2014; Zhang et al. 2016). In this study, we sequenced and described the first complete mitochondrial genome o
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