The complete mitochondrial genome of Gymnocypris przewalskii kelukehuensis (Teleostei: Cyprinidae)
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TECHNICAL NOTE
The complete mitochondrial genome of Gymnocypris przewalskii kelukehuensis (Teleostei: Cyprinidae) Chao Tong1,2,3,4 · Yongtao Tang1,2,3,4 · Kai Zhao1,2,3
Received: 29 December 2016 / Accepted: 17 February 2017 © Springer Science+Business Media Dordrecht 2017
Abstract Gymnocypris przewalskii kelukehuensis: (Teleostei: Cyprinidae) is a new subspecies of Gymnocypris przewalskii which endemic to Lake Keluke on the Tibetan Plateau,. However, it is now threatened by exotic species invasions and overfishing, and its natural population has been dramatically decreased. Here we firstly report its complete mitochondrial genome by low-coverage whole genome sequencing method. The total length of mitogenome is 16,682 bp, including 22 transfer RNAs (tRNAs), 13 protein-coding genes (PCGs) and two ribosomal RNAs (rRNAs). The putative control region is located between tRNA-Phe and tRNA-Pro, with an A + T content of 64.11%. The base composition of the genome is A (28.73%), C (25.86%), G (18.04%) and C (27.35%) with an overall GC content of 43.91%. Phylogenetic analysis suggests that G. p. kelukehuensis is closely related to G. przewalskii complex. * Kai Zhao [email protected] Chao Tong [email protected] Yongtao Tang [email protected] 1
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
2
Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
3
Qinghai Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
4
University of Chinese Academy of Sciences, Beijing 100049, China
Keywords Illumina sequencing · Gymnocypris przewalskii kelukehuensis · Subspecies · Mitogenome Gymnocypris przewalskii kelukehuensis is a newly identified subspecies of Gymnocypris przewalskii inhabited saline Lake Keluke on the Tibetan Plateau (Wu and Wu 1992), and revealed by both morphological and genetic evidences (Zhang et al. 2013). Human activities and exotic species invasions could drive declines of biodiversity in remaining intact ecosystem fragments and accelerating species extinctions (Ceballos et al. 2015). Indeed, overfishing and exotic species invasions (Cyprinus carpio, Hypophthalmichthys molitrix and Ctenopharyngodon idella) have dramatically deceased natural population size of G. p. kelukehuensis (Wu and Wu 1992; Zhang et al. 2013), which lead this endemic fish species to be endanger. To facilitate its genetic resource and thus contribute to its conservation, we first assembled and characterized its complete mitochondrial genome by high-throughput Illumina sequencing. Annotated mitogenomic sequence has been deposited into GenBank under the accession number KY406745. The total genomic DNA was extracted from muscle of G. p. kelukehuensis using Genomic DNA Isolation Kit (QiaGene, Germany) according to the manufacturer’s protoco
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