Anti- Helicobacter pylori activity of acomplex mixture of Lactobacillus paracasei HP7 including the extract of Perilla f
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Lee et al. Laboratory Animal Research (2020) 36:40 https://doi.org/10.1186/s42826-020-00073-x
RESEARCH
Open Access
Anti-Helicobacter pylori activity of acomplex mixture of Lactobacillus paracasei HP7 including the extract of Perilla frutescens var. acuta and Glycyrrhiza glabra Hyun-A Lee1, Joo-Yun Kim2, Jisoo Kim2, Bora Nam2 and Okjin Kim1*
Abstract The effect of standard therapeutic strategies on Helicobacter pylori infection is diminished over time owing to the emergence of drug resistant strains. In this study, we would like to confirm the enhanced effect of L. paracasei HP7, which has been reported to exert antibacterial and gastric mucosal protective effects, in combination with Perilla frutescens var. acuta (P. frutescens)and Glycyrrhiza glabra (G. glabra) extracts. P. frutescens extract and G. glabra extract were found to inhibit the growth of H. pylori in a concentrationdependent manner, and the combination of L. paracasei HP7 and P. frutescens extract and G. glabra extract effectively inhibited H. pylori from attaching to AGS a gastric epithelial cells. Moreover, L. paracasei HP7 complex mixture containing P. frutescens and G. glabra extracts has been shown to inhibit H. pylori virulence genes such as AlpA, CagA, FlaA and UreA. When H. pylori-infected mice were administered a complex mixture of L. paracasei HP7 containing P. frutescens and G. glabra extract, the infection rate of H. pylori was significantly reduced. In addition, the L. paracasei HP7 complex mixture significantly reduced serum IL-8 levels and stomach inflammation in H. pylori infected mice. These results suggest that a complex mixture of L. paracasei HP7 containing P. frutescens and G. glabra extracts may be an alternative to treating diseases caused by H. pylori infection. Keywords: Lactobacillus paracasei, HP7, Helicobacter pylori, Perilla frutescens var. acuta, Glycyrrhiza glabr
Introduction Helicobacter pylori, a major causative pathogen of chronic gastritis [1] and gastric ulcers [2], is a spiral of gram-negative bacteria associated with an increased risk of gastric cancer [3, 4]. Vaccination with antibiotics to remove gastric H. pylori can reduce H. pylori-associated gastrointestinal diseases [5, 6] and reduce the risk of gastric cancer [7]. The standard recommended therapy for H. pylori uses two antibiotics, usually a triple * Correspondence: [email protected] 1 Center for Animal Resources Development, Wonkwang University, 460 Iksandae-ro, Iksan 54538, Republic of Korea Full list of author information is available at the end of the article
combination therapy, including clarithromycin and a proton pump inhibitor with amoxicillin or metronidazole [8, 9]. However, the efficacy of the triple therapy has currently reduced over time. Recent cure rates of less than 80% are mainly due to the increased prevalence of resistant H. pylori strains in metronidazole and clarithromycin [10–12]. In addition, some patients showed allergic side effects to antibiotics and can sometimes cause side effects if H. pylori is not treated [13]. Longterm v
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