Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa
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BIOTECHNOLOGY AND INDUSTRIAL MICROBIOLOGY - RESEARCH PAPER
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa Diana F. Grigoletto 1 & Daniela B. B. Trivella 2 & André G. Tempone 3 & André Rodrigues 4,5 & Ana Maria L. Correia 4 & Simone P. Lira 1 Received: 27 May 2019 / Accepted: 7 April 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Fungi in the genus Trichoderma are notorious producers of secondary metabolites with diverse applications, such as antibacterial, antifungal, and plant growth-promoting properties. Peptaibols are linear peptides produced by such fungi, with more than 440 compounds described to date, including tricholongins, longibrachins, trichobrachins, and trichovirins. Peptaibols are synthesized by non-ribosomal peptide synthetases and they have several biological activities. Our research group isolated four peptaibols (6DP2, 6DP3, 6DP4, and 6DP5) with antifungal activity against the plant pathogen Colletotrichum gloeosporioides and the proteasome (a cancer chemotherapy target) from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa. The ethyl acetate extract of this endophyte showed activity of 6.01% and 75% against C. gloeosporioides and the proteasome, respectively. The isolated compounds were identified by MS/MS and compared to literature data, suggesting the presence of trilongins BI, BII, BIII, and BIV, which are peptaibols containing 20 amino acid residues. The minimum inhibitory concentration against C. gloeosporioides was 40 μM for trilongin BI, 320 μM for trilongin BII, 160 μM for trilongin BIII, and 310 μM for trilongin BIV. BI–BIV trilongins inhibited proteasome ChTL activity, with IC50 values of 6.5 ± 2.7; 4.7 ± 1.8; 6.3 ± 2.2; and 2.7 ± 0.5 μM, respectively. The compounds were tested ex vivo against the intracellular amastigotes of Leishmania (L.) infantum but showed no selectivity. It is the first report of trilongins BI–BIV with antifungal activity against C. gloeosporioides and the proteasome target. Keywords Colletotrichum gloeosporioides . Alcatrazes Island . Peptaibiotics . Peptaibols . Proteasome
Introduction Responsible Editor: Luis Henrique Souza Guimaraes. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42770-020-00270-9) contains supplementary material, which is available to authorized users. * Simone P. Lira [email protected] 1
Departamento de Ciências Exatas, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, CP 9, Piracicaba, SP 13418-900, Brasil
2
Centro Nacional de Pesquisa em Energia e Materiais, Laboratório Nacional de Biociências, CP 6192, Campinas, SP 13083-970, Brasil
3
Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, São Paulo, SP 01246-000, Brasil
4
Departamento de Biologia Geral e Aplicada, Universidade Estadual Paulista (UNESP), Rio Claro, SP 13506-900, Brasil
5
Centro de Estudos de Insetos Sociais, Universidade Estadual Paulista (UNESP), Rio Claro, SP 13506-900, Brasil
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