Identification of an endophytic fungus Pilidiella guizhouensis isolated from Eupatorium chinense L . and its extracellul
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ORIGINAL ARTICLE
Identification of an endophytic fungus Pilidiella guizhouensis isolated from Eupatorium chinense L. and its extracellular polysaccharide Jianfen Zhang 1 & Bo Yang 2 & Hong Chen 1 Received: 13 December 2019 / Accepted: 27 February 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract An endophytic fungus strain ZJSRU-M1 was isolated from Eupatorium chinense L. The phylogenetic tree analysis indicated that the ITS sequence of ZJSRU-M1 was similar to that of Pilidiella guizhouensis. Therefore, the endophytic fungus was identified as Pilidiella guizhouensis. The strain could produce a lot of exopolysaccharides in the process of liquid fermentation. The exopolysaccharide from the submerged culture of P. guizhouensis ZJSRU-M1 (PGP) was purified. Its molecular weight was determined by HPGPC with the average molecular weight of 13.4 kDa. The monosaccharide composition of PGP was determined by GC–MS, indicated that it was a homogeneous polysaccharide, consisted of glucose only. And PGP was detected by FT-IR spectroscopy and UV spectroscopy, suggested that it was a polysaccharide structure. In addition, the antioxidant activity of PGP in vitro, including scavenging ability on DPPH radical, hydroxyl radical and superoxide anion, suggested that PGP had strong antioxidant activity. These findings suggested that exopolysaccharide from endophytic fungus P. guizhouensis had the potential application for the development of antioxidant drugs or health products. Keywords Pilidiella guizhouensis . Exopolysaccharide . Endophytic fungus . Eupatorium chinense L
Abbreviations PGP The exopolysaccharide from the submerged culture of P. guizhouensis ZJSRU-M1 HPGPC High performance gel permeation chromatography GC–MS Gas chromatography-mass spectrometer UV Ultraviolet FT-IR Fourier transform infra-red spectroscopy DPPH 1,1-diphenyl-2-picrylhydrazyl Vc Ascorbic acid
* Jianfen Zhang [email protected] 1
College of Biology and Environmental Engineering, Zhejiang Shuren University, No. 8 Shuren Street, Hangzhou 310015, China
2
College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 260 Baichuan Street, Hangzhou 311402, People’s Republic of China
Introduction Eupatorium chinense L. is an annual or perennial herb of the genus Eupatorium in Compositae (Liu et al. 2015). It mainly distributes in the southeastern to southwestern provinces in China. Traditional pharmacological studies have shown that Eupatorium chinense L. has the effects of clearing away heat and detoxification, cooling blood and promoting pharynx, anti-inflammation and analgesia (Liu et al. 2015; Yang et al. 2004). In recent years, studies have shown that Eupatorium chinense L. is rich in terpenoids and flavonoids with novel structures and has significant antimicrobial and antitumor activities (Liu et al. 2015; Yang et al. 2004). Endophytic fungi are a group of microorganisms that have been infected asymptomatically, flourish in the healthy tissues of the host, with no any apparent symptoms of disease (Stone et al
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