Applications of Continuous and Steady-State Methods to Root Biology
This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding of
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Applications of Continuous and Steady-State Methods to Root Biology Edited by
JOHN G. TORREY Harvard Forest, Petersham, Massachusetts 01366, USA
and
LAWRENCE J. WINSHIP 5J'choal arNall/ral Sciences, Hampshire College, Amherst, Massachusetts 0/002, USA
KLUWER ACADEMIC PUBLISHERS DORDRECHT / BOSTON / LONDON
Lihrary of Congress Cataloging in Puhlication Data Appllcatl0ns of continuous and steady-state methods to root biology / edited by John G. Torrey. Lawrence J. Winship. p. cm. -- (Developments in plant and soil sciences) Includes indexes. 1. Roots (Botany)--Research. 2. Botany--Technique. I. Torrey. John G. II. Winship. Lawrence J .• 1952III. Series. QK644.A67 1989 88-29415 582' .0498--dc19
CIP
ISBN-13: 978-94-010-7502-2 e-ISBN-13: 978-94-009-2237-2 DOl: 10.1007/978-94-009-2237-2
Puhlished by Kluwcr Academic Publishers. P.O. Box 17.3300 AA Dordrecht. The Netherlands. Kluwer Academic Publishers incorporates the publishing programmes of D. Reidel. Martinus Nijhoff, Dr W. Junk and MTP Press. Sold and distrihuted in the U.S.A. and Canada by Kluwer Academic Puhlishers. 101 Philip Drive, Norwell, MA 02061. U.S.A. In all other countries, sold and distributed by Kluwer Academic Publishers Group. P.O. Box 322. 3300 AH Dordrecht, The Netherlands.
printed Oil acid ji'ee paper
All Rights Rescrved © 1989 by Kluwer Academic Publishers Softcover reprint of the hardcover 1st edition 1989
No part of the material protected by this copyright notice may be reproduced or utilized in any form or hy any means. electronic or mechanical including photocopying, recording or by any information storage and retrieval system. without written permission from the copyright owner.
Table of Contents Foreword
IX
Introduction
xi
Acknowledgements
xv
Chapter 1. A Multichannel System for Steady-State and Continuous Measurements of Gas Exchanges from Legume Roots and Nodules D.B. LA YZELL, S. HUNT, B.J. KING, K.B. WALSH and G.E. WEAGLE 1. Introduction 2. Overview of the Gas Exchange System 3. Application of the Gas Exchange System to Studies of Legume Physiology 4. Conclusions Chapter 2. Quantification of Diffusion Characteristics in Spherical Nodules: A Comparison of Methods P.R. WEISZ and T.R. SINCLAIR 1. Introduction 2. Homogeneous Model of Nodule Diffusion 3. Diffusion Barrier Models 4. Comparisons Among Diffusion Barrier Models 5. Conclusions 6. Appendix. Sensitivity of Transient Analysis to Changes in Vt/n
1
3 20 24 29 29 30 33 46 48 49
Chapter 3. Simple Apparatus for Growth of Nodulated Plants and for Continuous Nitrogenase Assay Under Defined Gas Phase W.B. SILVESTER, R. PARSONS, F.R. MINCHIN and J.F. WITTY 1. Introduction 2. Apparatus - Plant Growth in Defined O 2 Levels 3. Apparatus - Assays in Open, Flow-Through Systems Chapter 4. Continuous Measurements of Nitrogenase Activity in the Study of Environmental Responses of Frankia and Other Bacteria W.B. SILVESTER and L.J. WINSHIP 1. Introduction 2. Materials and Methods 3. Results 4. Discussion v
55 55 56 59
67 67 68 69 76
VI
Chapter 5. Limitations and Errors in Gas