Root Development Methods and Protocols

This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers me

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Daniela Ristova Elke Barbez Editors

Root Development Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Root Development Methods and Protocols

Edited by

Daniela Ristova Gregor Mendel Institute of Molecular Plant Biology Vienna, Wien, Austria

Elke Barbez Institute of Applied Genetics & Cell Biology University of Natural Resources and Life Sciences Vienna, Wien, Austria

Editors Daniela Ristova Gregor Mendel Institute of Molecular Plant Biology Vienna, Wien, Austria

Elke Barbez Institute of Applied Genetics & Cell Biology University of Natural Resources and Life Sciences Vienna, Wien, Austria

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7746-8 ISBN 978-1-4939-7747-5 (eBook) https://doi.org/10.1007/978-1-4939-7747-5 Library of Congress Control Number: 2018934877 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface The root system, the part of plants hidden in the soil, represents an essential organ for plant growth and productivity. Root development exhibits a high level of developmental plasticity, continuously translating environmental information into developmental decisions. Environmental changes have become an increased challenge for crop production. Therefore, deciphering root developmental mechanisms in detail will be crucial for understanding how plants cope with environmental changes and how to translate the fundamental knowledge into