Assessment and Comparison of the Overall Analytical Potential of Capillary Electrophoresis and High-Performance Liquid C
- PDF / 6,073,657 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 45 Downloads / 220 Views
ORIGINAL
Assessment and Comparison of the Overall Analytical Potential of Capillary Electrophoresis and High‑Performance Liquid Chromatography Using the RGB Model: How Much Can We Find Out? Paweł Mateusz Nowak1 · Edyta Sekuła1 · Paweł Kościelniak1 Received: 18 March 2020 / Revised: 26 June 2020 / Accepted: 3 July 2020 © The Author(s) 2020
Abstract The choice between capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) is not easy and depends on many factors. An attempt to facilitate this choice is this work, in which both techniques have been confronted on the basis of the RGB model, offering a transparent and pictorial way to compare individual parameters as well as the overall analytical potential of each tool. To ensure the universal nature of the comparison, a simple in composition and chemically diverse model sample was used, accompanied by the data processing method reducing the potential impact of analyte selection. Moreover, permanent coating of the inner surface of the capillary and addition of a surfactant to the separation buffer were considered as the additional factors that may affect the assessment of the CE technique. The presented analysis can be valuable in any discussions about the intrinsic advantages and disadvantages of CE and HPLC, and divagations on how they affect the overall potential and usability of each. We also provide access to the Excel worksheets used for the assessment, which can be easily modified to reevaluate the methods with a different selection of variables, and analyze other possible scenarios. Keywords Capillary electrophoresis · High-performance liquid chromatography · Capillary coating · Micellar electrokinetic chromatography · RGB model
Introduction Capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are powerful and recognized in the analytical world separation techniques [1, 2], with different specifications, often mutually competitive due to different advantages and disadvantages. Despite the differences in the separation mechanism, in many cases, the separation of given groups of analytes is possible with good efficiency using both techniques. In such situations, choosing a better one in overall is not easy and requires an in-depth and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10337-020-03933-9) contains supplementary material, which is available to authorized users. * Paweł Mateusz Nowak [email protected] 1
Faculty of Chemistry, Department of Analytical Chemistry, Jagiellonian University in Kraków, Gronostajowa St. 2, 30‑387 Kraków, Poland
critical look. Any attempt to transparently compare their overall potential and usability requires analyzing various criteria, selecting them, assigning appropriate weights, and proposing an appropriate evaluation algorithm. The situation is complicated by the fact that in addition to the standardized validation criteria related to the quality of analytical results, the overall potential of the method is als
Data Loading...
