Assessment of circulating miR-20b, miR-221, and miR-155 in occupationally lead-exposed workers of North-Western India
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RESEARCH ARTICLE
Assessment of circulating miR-20b, miR-221, and miR-155 in occupationally lead-exposed workers of North-Western India Prasenjit Mitra 1 & Taru Goyal 1 & Preeti Singh 1 & Shailja Sharma 1 & Praveen Sharma 1 Received: 19 May 2020 / Accepted: 30 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Lead (Pb), a toxic heavy metal, is capable of inducing several adverse health effects following its accumulation in the body. Lead is a potential carcinogen, capable of causing multisystem alterations. Recent reports identify small regulatory RNA molecules— miRNAs—which show differential expression in individuals exposed to similar levels of lead. These miRNAs can become potential molecular biomarkers of lead toxicity in the future and may unravel the possible molecular pathways through which this metal may exert its toxic manifestations. The present study aimed to assess the circulating levels of miRNA-20b, 221, and 155 in occupationally lead-exposed workers and correlate them with blood lead levels. One hundred ten participants working in various factories of Jodhpur and 97 participants not occupationally exposed to lead were recruited after obtaining due informed consent. Blood lead level (BLL) was estimated by graphite furnace atomic absorption spectrophotometry (GF-AAS). Circulating miRNAs were isolated from serum by Qiagen miRNA isolation kit and converted to cDNA by commercial kit. Expression profiles of miR20b, miR-221, and miR-155 were performed in RT-PCR using Qiagen miRNA PCR assays. The blood lead level (mean ± SD) of occupationally lead-exposed subjects was 6.94 ± 11.96 μg/dL while that of non-exposed was 2.39 ± 4.66 μg/dL. Out of the three miRNAs, miR-155 and miR-221 were significantly upregulated, while miR-20b did not show significant difference among study groups. The fold change of miR-20b, miR-221 and miR-155 expression were 1.08, 2.71 and 2.07 respectively. Functional analysis revealed that these miRNAs have the potential to trigger various genes and cellular pathways. The findings of our study highlight the importance of miRNA dysregulation in lead-exposed individuals that may contribute to the systemic effects of lead toxicity. Keywords BLL . miRNA . Occupational exposure . Biomarker
Introduction Lead (Pb), a common heavy metal, is widely used in various industries. Mining and metal smelting are the two most important artificial sources of lead emission (Castro et al. 2009; Longatto-Filho et al. 2009). Being a classical environmental toxic metal, it has the potential to affect multiple organ systems of the human body, like nervous, hematopoietic, urinary, gastrointestinal, and even reproductive systems. The toxic Prasenjit Mitra and Taru Goyal contributed equally to this work. Responsible editor: Lotfi Aleya * Praveen Sharma [email protected] 1
Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India
effect of lead exposure is highly diverse and depends on many factors like target organ, route of exposure, t
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