AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions
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GENETICS & EVOLUTIONARY BIOLOGY - ORIGINAL ARTICLE
AteqTERT expression and specific tissue activity in a 2‑year‑old complete plant in Agave tequilana in field conditions Z. De la Torre Espinosa1,2 · Emmanuel Ávila De Dios3 · Felipe Sánchez Teyer2 · Enrique Castano1 Received: 3 March 2020 / Revised: 27 July 2020 / Accepted: 29 July 2020 © Botanical Society of Sao Paulo 2020
Abstract Telomerase is a specialized enzyme that attributes to maintaining and lengthening of telomeric length as its primary function. This ribonucleoprotein complex requires two minimum subunits for its in vivo function: the TERT amino acid subunit and TER its ribonucleic subunit. Crystallographic studies have shown that stable interaction between subunits and their domains is essential during the active phase. Plants and humans have shown to be tissue specific and dependent on cell proliferation. In Arabidopsis thaliana (L.) Heynh, the expression of AtTERT was determined to be necessary for activity. The objective of this work is to perform the in silico characterization of TERT in Agave tequilana F.A.C. Weber and evaluate the expression of TERT and the specific tissue activity in 2-year-old plants. To this end, we use the transcriptome library of A. tequilana (Ávila de Dios unpublished data) in TPM and qTRAP. We observe in this work that the consensus sequence shows all the specific domains of the TERT subunit. Additionally, we carried out the in silico prediction of the structural model of AteqTERT. The model presents the characteristic TERT ring of the crystallized models in active state of telomerase, biological annotations were observed, such as heavy metals M g2+, NUC and CH1, and finally we note that, there is no correlation between TERT expression in TPM and telomerase activity in 2-year meristematic tissue. Keywords In silico characterization · Quantitative activity of telomerase (qTRAP) · Three-dimensional protein modeling · Trascriptomic assembly library (TSA)
1 Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40415-020-00642-3) contains supplementary material, which is available to authorized users. * Enrique Castano [email protected] 1
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, Mexico
2
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, Mexico
3
Unidad de Ingeniería Genética, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Km 9.6 Libramiento Norte Carretera Irapuato – León, Irapuato, Guanajuato, Mexico
Telomerase is a specialized enzyme necessary for the maintenance of telomeric length, and consequently, it is essential to provide genomic stability in the cell (Wyatt et al. 2010; Kim and Kim 2018). Telomerase can have two different roles of action, such as bind to telomeric sequences for telomeric leng
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