Ethanol Production from Enzymatic Hydrolysates Optimized of Agave tequilana Weber var. azul and Agave karwinskii bagasse
- PDF / 1,369,042 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 48 Downloads / 192 Views
Ethanol Production from Enzymatic Hydrolysates Optimized of Agave tequilana Weber var. azul and Agave karwinskii bagasses M. E. Delfin-Ruíz 1 & M. Calderón-Santoyo 1 & J. A. Ragazzo-Sánchez 1 & J. Gómez-Rodríguez 2 & M. G. Aguilar-Uscanga 2 Received: 8 April 2020 / Accepted: 23 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The use of biofuels obtained from lignocellulosic materials is a technology that offers great potential for their production in the future. Agave bagasse is one of the most common wastes produced in Mexico through the Syrup, Tequila, and Mezcal industry. In particular, Tequila and Mezcal are one of the largest alcoholic beverages in the country and thousands of tons of this waste are produced annually. Therefore, agave bagasse is a promising alternative in Mexico to obtain second-generation bioethanol (2G). In this work, the use of enzymatic hydrolysates obtained by an alkaline pretreatment and enzymatic hydrolysis optimized using Agave tequilana Weber var. azul bagasse (AT) and Agave karwinskii bagasse (AK) was evaluated for ethanol production. For this, the optimization design of alkaline pretreatment and enzymatic hydrolysis was carried out using a Box-Behnken design. Both hydrolysates were fermented using Saccharomyces cerevisiae ITV01 yeast. The results showed that the best lignin reduction in the alkaline pretreatment was 77.6 and 84.29% utilizing AT and AK, respectively. Furthermore, a glucose concentration of 100.06–98.08 and 64.03–60.0 g/L was found in the enzymatic hydrolysis (AT and AK, respectively), reaching in the fermentation step a yield of 0.47 and 0.46 g/g, volumetric productivity of 1.97 and 1.14 g/L/h and a fermentation efficiency of 93.1 and 90.4% using AT and AK, respectively. These results are interesting to use agave bagasse for ethanol production in Mexico as an alternative to give added value and take advantage of its availability. Keywords Biomass . Biofuels . Pretreatment . Fermentation . Box-Behnken . S. cerevisiae
Introduction With the growing interest in the production of biofuels, different lignocellulosic materials have been studied in order to obtain high yields, which is an economically feasible product [1–4]. The use of agave bagasse as a feedstock for ethanol production has been studied due to the abundance of this material in Mexico and for its use in the production of alcoholic beverages such as Tequila, Mezcal, Pulque, and Bacanora [5–9]. These residues are mainly composed of hemicellulose, cellulose, and lignin, and because they can be used in the production of second-generation ethanol, it is necessary to
* M. G. Aguilar-Uscanga [email protected]; [email protected] 1
Tecnológico Nacional de Mexico/I. T. Tepic, Laboratorio Integral de Investigación en Alimentos, CP. 63175 Tepic, Nayarit, México
2
Tecnológico Nacional de México/I. T. Veracruz, Departamento de Ing. Química y Bioquímica, Unidad de Investigación y Desarrollo en Alimentos (UNIDA), Czda. M. A. de Quevedo Núm. 2779, C.P. 91860 Vera
Data Loading...
