Biopolymer Hydrogels Regenerated From Agave Tequilana Waste For Cytocompatable Materials
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Biopolymer Hydrogels Regenerated From Agave Tequilana Waste For Cytocompatable Materials Takaomi Kobayashi1, Karla L. Tovar-Carrillo 1,2, Kazuki Nakasone1, Motohiro Tagaya1 Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, Japan, 2 Departamento de Ciencias Químico-Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juarez 32300, Chihuahua,Mexico. 1
ABSTRACT Agave fibers were used to elaborate a transparent and flexible cellulose hydrogel films used as scaffold for tissue regeneration and tested by in vitro assays with NIH 3T3 fibroblast cells. Using dimethylacetamide/lithium chloride (DMAc/LiCl) system was possible to obtain cellulose solutions and hydrogel films were prepared by phase inverse method without cross-linker. The concentration of LiCl in the DMAc solution was varied from 4 to 12 wt% in the phase inversion process and then the cytotoxicity was tested for 14 days on the cultivation. The resultant hydrogel films showed better cytocompatibility than the PS dish used as control. The cell growing images showed that the hydrogel films with lower LiCl apparently contained ordered and aggregated fiber orientation. This comparison suggested that the segmental microstructure in the hydrogel films influenced fibroblast cells spreading. In addition, the agave hydrogel films displayed good stability without biodegradiation through the cell cultivation. INTRODUCTION Hydrogels have been the primarily choice for a large number of researchers for many applications in regenerative medicine due to their unique biocompatibility [1,2]. Such attractive work shows that hydrogels can serve as scaffolds and provide structural integrity to tissue constructs [3-5]. In time, researchers have noted that synthetic polymers have offered limited properties for biological cell growing on the scaffold.Unfortunately this scaffold type still could not provide an ideal environment to support cell adhesion and tissue formation due to their bioinert nature. In our strategy for biopolymer, cellulose having abundant hydroxyl groups, pure hydrogels of cellulose are tried to fascinating for the use of scaffold on fibroblast tissue engineering. Agave Tequilana waste fibers were used as biocellulosesource to elaborate hydrogel films [6,7]. Moreover, agave tequilana Weber azul, is an economically important plant cultivated in central Mexico for the production of tequila. More than 800 thousand tons of agave bagasses have been disposed as waste product per year [8]. This becomes as an important problem for the disposal of the bagasse agave and has been attention to regeneration to useful material. Within these waste products, bagasse from Agave tequilana Weber azul is found that until now, several research works have been performed to offer an alternative use for this waste product, while none is offered us a final solution, due to the necessity of the development of new technologies to solve this problem[9]. In our work [10], it was found that using dime
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