Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promot
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Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion Karina A. Balderas‑Ruíz1, Patricia Bustos2, Rosa I. Santamaria2, Víctor González2, Sergio Andrés Cristiano‑Fajardo1, Salvador Barrera‑Ortíz3, Miriam Mezo‑Villalobos4, Sergio Aranda‑Ocampo5, Ángel Arturo Guevara‑García3, Enrique Galindo1 and Leobardo Serrano‑Carreón1*
Abstract Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment of this strain as BCA (biological control agent), as well as PGPB (plant growth-promoting bacteria), were demonstrated through in vivo and in vitro assays. In vivo assays showed that B. velezensis 83 was able to con‑ trol anthracnose (Kent mangoes) as efficiently as chemical treatment with Captan 50 PH™ or Cupravit hidro™. The inoculation of B. velezensis 83 to the roots of maize seedlings yielded an increase of 12% in height and 45% of root biomass, as compared with uninoculated seedlings. In vitro co-culture assays showed that B. velezensis 83 promoted Arabidopsis thaliana growth (root and shoot biomass) while, under the same experimental conditions, B. velezensis FZB42 (reference strain) had a suppressive effect on plant growth. In order to characterize the isolated strain, the com‑ plete genome sequence of B. velezensis 83 is reported. Its circular genome consists of 3,997,902 bp coding to 3949 predicted genes. The assembly and annotation of this genome revealed gene clusters related with plant-bacteria interaction and sporulation, as well as ten secondary metabolites biosynthetic gene clusters implicated in the biologi‑ cal control of phytopathogens. Despite the high genomic identity (> 98%) between B. velezensis 83 and B. velezensis FZB42, they are phenotypically different. Indeed, in vitro production of compounds such as surfactin and bacillomycin D (biocontrol activity) and γ-PGA (biofilm component) is significantly different between both strains. Keywords: Bacillus velezensis 83, Genome sequencing, Biological control agent, PGPB, Secondary metabolites production Keypoints B. velezensis 83 is a biological control agent and plant growth-promoting bacteria. Phylogenomic analysis allowed to reclassify B. subtilis 83 as B. velezensis 83. B. velezensis 83 has genes to establish a rhizosphere plantbacteria interaction.
*Correspondence: [email protected] 1 Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad #2001, Col. Chamilpa, C. P. 62210 Cuernavaca, Morelos, México Full list of author information is available at the end of the article
Introduction Bacillus is a bacterial genus which comprises several species that establish, directly or indirectly, beneficial relations with plants (Olanrewaju et al. 2017). By direct mechanisms some Bacillus strains promote plant growth, by improving the acquisition of nutrients such as nitrogen and phosphate (biofertilization), or through
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