Bacterial cellulose nanofiber distribution on gelatin and silk fibroin scaffolds and the cell behavior
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ORIGINAL RESEARCH
Bacterial cellulose nanofiber distribution on gelatin and silk fibroin scaffolds and the cell behavior Zhuotong Wu . Yu Jiang . Zhe Li . Baoxiu Wang . Huaping Wang . Shiyan Chen
Received: 21 July 2020 / Accepted: 19 October 2020 Ó Springer Nature B.V. 2020
Abstract This paper presents a simple strategy to adjust bacterial cellulose (BC) nanofiber distribution on the biomimetic 3D scaffolds by in situ bacteria culture on the protein scaffolds which were prepared by selecting gelatin (Gel) with positive charge and silk fibroin (SF) with negative charge. The protein scaffolds with different structures and properties were obtained by adjusting the proportion of Gel and SF. Due to the electrical attraction and repulsion of bacteria and protein, the density and distribution of bacteria on the protein scaffolds is obviously different. Thus, after in situ culture, protein/BC scaffolds with different nanofibers density and distribution from basically adhering to the pore wall to distributing in the middle pore were obtained, which make different
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03545-4) contains supplementary material, which is available to authorized users. Z. Wu Y. Jiang B. Wang H. Wang S. Chen (&) State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, People’s Republic of China e-mail: [email protected] Z. Li (&) College of Materials and Textile Engineering, Jiaxing University, Jiaxing 314001, Zhejiang, People’s Republic of China e-mail: [email protected]
cell shape and distribution. It is can be easily realized by adjusting the properties of scaffold materials to guide the growth of bacteria which provides a simple idea for the design of tissue engineering scaffolds applied in medical implants, cell supports and other tissue regeneration. Keywords Gelation and silk fibroin scaffolds Bacterial cellulose Distribution Cell behavior
Introduction Tissue engineering has been successfully used to construct human tissues and organs, such as skin, bone, tendon, blood vessel, heart valve, etc. (Duan 2017; Hollister 2005; Zaborowska et al. 2010). Scaffold material, which plays a key role in tissue construction, is one of the most important roles in tissue engineering. The extracellular matrix (ECM) obtained from natural tissue by acellularization can provide the best microenvironment for cell growth, which is an ideal scaffold for tissue engineering, but the source of such materials is limited (von der Mark et al. 2009). Among the currently known natural fibers, the nano-network structure of BC is similar to ECM (Bodin et al. 2010; Kim et al. 2013; Rajwade et al. 2015). The design of BC-based tissue engineering scaffolds has also attracted the attention of researchers
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Cellulose
(Andersson et al. 2010; Chiaoprakobkij et al. 2011). However, the dense structure of
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