Bacterial Regulatory RNA Methods and Protocols
This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their diffe
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Véronique Arluison Claudio Valverde Editors
Bacterial Regulatory RNA Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Bacterial Regulatory RNA Methods and Protocols
Edited by
Véronique Arluison Université Paris Diderot & LLB/CEA/CNRS, Gif-sur-Yvette, France
Claudio Valverde Laboratorio de Bioquímica, Microbiología e Interacciones, Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes-CONICET, Bernal, Argentina
Editors Véronique Arluison Université Paris Diderot & LLB/CEA/CNRS Gif-sur-Yvette, France
Claudio Valverde Laboratorio de Bioquímica Microbiología e Interacciones Biológicas en el Suelo Departamento de Ciencia y Tecnología Universidad Nacional de Quilmes-CONICET Bernal, Argentina
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7633-1 ISBN 978-1-4939-7634-8 (eBook) https://doi.org/10.1007/978-1-4939-7634-8 Library of Congress Control Number: 2017964485 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Regulatory small noncoding RNAs (sRNAs) are ubiquitous key regulators of gene expression in prokaryotes, operating at the post-transcriptional level to influence the fate of mRNA translation and/or stability, in most cases with the complicity of RNA binding proteins. Although significant progress has been made in the past 20 years to understand the function of individual sRNAs, high-throughput R
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