Bioconversion of Agro-waste to Value-Added Product Through Solid-State Fermentation by a Potent Fungal Strain Aspergillu
Laccases are multicopper-containing oxidoreductase enzymes, which are commonly produced by plants and microbes, are involved in various biotechnological applications like bleaching of textile dyes, biofuels, biosensors, detoxification of effluent, bioreme
- PDF / 437,819 Bytes
- 9 Pages / 439.37 x 666.142 pts Page_size
- 53 Downloads / 125 Views
*) Department of Food Technology & Biochemical Engineering, Jadavpur University, 700032 Kolkata, West Bengal, India e-mail: [email protected]; [email protected] © Springer Nature Singapore Pte Ltd. 2018 S.K. Ghosh (ed.), Utilization and Management of Bioresources, DOI 10.1007/978-981-10-5349-8_28
291
292
P. Ghosh and U. Ghosh
1 Introduction Laccases are multicopper-containing enzymes that are able to reduce molecular oxygen to water and instantaneously perform one-electron oxidation of various aromatic substrates such as diphenols, methoxy-substituted monophenols, and aromatic amines. For the last few decades, it has gained significant interests due to their potentiality for oxidizing phenolic and nonphenolic lignin-related compounds and different recalcitrant environmental pollutants. Currently in different fields like delignification of pulp, textile dye bleaching, effluent detoxification, biotransformation of antibiotics and steroids, development of biosensors, etc. (Chhaya and Gupte 2010). For better application biotechnologically, the yield of the enzyme should be high enough, and the production cost should be low. Considering this, solid-state fermentation (SSF) can be a better option, where different agro-wastes could be effectively utilized in enzyme production through microbial fermentation. In agro- industries, large quantity of agricultural residues accumulates. Disposal of these agro-wastes is one of the serious problems for industries. This condition could be partially resolved through bioconversion of these materials to value-added products through microbial fermentation. This is a promising technology because it has different beneficial aspects like lower-energy requirements, high product yield, and less infrastructural requirements and is eco-friendly. Therefore, SSF could be a promising alternate for valorization of agro-wastes to value-added products like laccases.
2 Materials and Methods 2.1 Microorganism and Inoculum Preparation Aspergillus flavus PUF5, a pre-isolated fungal strain, was used in the study. It was grown on potato dextrose agar (composition: potato extract 20 g, dextrose 2 g, agar 1.75%) slants at 30 °C for 5 days and stored at 4 °C until further use. About 5 ml of sterile distilled water was added to a fully sporulated culture slant. The spores were dislodged properly. A spore suspension of about to 5 × 109 spores/ ml was considered as inoculum for further studies.
2.2 Laccase Production Through Solid-State Fermentation Five grams of dried ridge gourd peel was used as substrates for laccase production by isolated fungal strain Aspergillus flavus PUF5. The buffer-moistened substrates were sterilized and incubated. Finally the crude enzyme was extracted, and the supernatants were assayed for laccase activity.
Bioconversion of Agro-waste to Value-Added Product Through Solid-State…
293
2.3 Optimization of Fermentation Parameters For maximum laccase production by Aspergillus flavus PUF5-utilizing waste ridge gourd peel, different fermentation parameters
Data Loading...
