n -Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products
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BIOTECHNOLOGY AND INDUSTRIAL MICROBIOLOGY - RESEARCH PAPER
n-Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products Bruno A. S. Santos 1 & Suéllen P. H. Azambuja 1 & Patrícia F. Ávila 1 & Maria Teresa B. Pacheco 2 & Rosana Goldbeck 1 Received: 9 October 2019 / Accepted: 25 August 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract n-Butanol is a renewable resource with a wide range of applications. Its physicochemical properties make it a potential substitute for gasoline. Saccharomyces cerevisiae can produce n-butanol via amino acid catabolic pathways, but the use of pure amino acids is economically unfeasible for large-scale production. The aim of this study was to optimize the production of n-butanol by S. cerevisiae from protein-rich agro-industrial by-products (sunflower and poultry offal meals). By-products were characterized according to their total protein and free amino acid contents and subjected to enzymatic hydrolysis. Protein hydrolysates were used as nitrogen sources for the production of n-butanol by S. cerevisiae, but only poultry offal meal hydrolysate (POMH) afforded detectable levels of n-butanol. Under optimized conditions (carbon/nitrogen ratio of 2 and working volume of 60%), 59.94 mg/L of n-butanol was produced using POMH and glucose as substrates. The low-cost agro-industrial by-product showed great potential to be used in the production of n-butanol by S. cerevisiae. Other protein-rich residues may also find application in biofuel production by yeasts. Keywords Saccharomyces cerevisiae . Biofuel . Nitrogen source . Optimization
Introduction Global concern about climate change and the need to replace fossil fuels have motivated the scientific community to search for environmentally friendly energy sources [1]. Biofuels are a promising substitute for fossil fuels because they can be produced by fermentation of renewable materials [2]. Large-scale ethanol production has become possible due to technological advances. Although it is the most widely used biofuel today, ethanol is not considered the best substitute for gasoline. Butanol, a higher alcohol with four carbons and four isomers Responsible Editor: Eleni Gomes. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42770-020-00370-6) contains supplementary material, which is available to authorized users. * Rosana Goldbeck [email protected] 1
Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil
2
Applied Chemistry and Nutrition Center, Institute of Food Technology (ITAL), Campinas, SP, Brazil
(n-butanol, sec-butanol, isobutanol, and tert-butanol) has some important advantages over ethanol [3]. In comparison with ethanol, butanol has higher blending ability with gasoline and energy density and is less volatile and more hydrophobic. Its main disadvantage is the low yield of fermentation reactions [4, 5]. Microorganisms of the genus Clostr
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