Biogenic zinc oxide, copper oxide and selenium nanoparticles: preparation, characterization and their anti-bacterial act
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ORIGINAL RESEARCH
Biogenic zinc oxide, copper oxide and selenium nanoparticles: preparation, characterization and their anti‑bacterial activity against Vibrio parahaemolyticus D Vinu1 · K. Govindaraju1 · R. Vasantharaja1 · S. Amreen Nisa1 · M. Kannan2 · K. Vijai Anand3 Received: 5 July 2020 / Accepted: 29 October 2020 © Islamic Azad University 2020
Abstract The present study investigates biogenic preparation of zinc oxide (ZnO), copper oxide (CuO) and selenium (Se) nanoparticles using the marine brown alga Sargassum swartzii. The prepared nanomaterials were characterized using X-ray diffraction pattern (XRD), scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM) and UV–Vis diffuse reflectance spectroscopy (DRS-UV) analysis. The particle size of biogenic ZnO, CuO and Se nanoparticles was ca. 32, 32 and 21 nm, respectively. The isolation of bacterial pathogenic strain Vibrio parahaemolyticus (V. parahaemolyticus) from the diseased shrimp and virulent genes (toxR and tlh) confirmed by PCR technique. Further, the molecular characterized using 16S ribosomal RNA gene sequences and identified new strain V. parahaemolyticus strain (GRCORNICRA001). Anti-bacterial activity of biogenic nanomaterials (ZnO, CuO and Se) was investigated against isolated V. parahaemolyticus using well diffusion method and growth inhibitory assay. The minimum inhibitory concentration (MIC) was 25, 25 and 10 µg mL−1 of ZnO, CuO and Se nanoparticles, respectively. The results show that there is a strong bacterial inhibition in a dose-dependent manner. Further, SEM analysis revealed that the interaction of nanomaterials with V. parahaemolyticus, resulted in a surface tension change that leads to membrane depolarization, formation of abnormal textures such as membrane rupture, membrane blebs, membrane clumping, and also caused cell death. Results of this effort highlighted the way for the future that these nanomaterials incorporated with shrimp feed for the management of aquatic diseases. Graphic abstract
Keywords Seaweed · Nanomaterials · 16S ribosomal RNA sequences · Anti-bacterial activity · SEM Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40097-020-00365-7) contains supplementary material, which is available to authorized users. Extended author information available on the last page of the article
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Vol.:(0123456789)
Journal of Nanostructure in Chemistry
Introduction Aquaculture is one of the largest food producing sectors across the globe. Disease is considered to be one of the major limiting factors in aquaculture production worldwide. It is witnessed that the aquaculture industry particularly, shrimp culture has been continuously suffered by various diseases that are caused by bacteria and viruses leading to high mortality rate, and huge economic loss [1]. Among pathogens, bacteriae belonging to family Vibrionaceae are the most significant and cause for high mortality rate in shrimp industry worldwide.
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